脂肪组织单细胞转录组分析方法。
Methods for Single Cell Transcriptomic Analysis of Adipose Tissue.
机构信息
Department of Molecular Pathobiology, New York University College of Dentistry, New York, NY, USA.
Department of Cell Biology, New York University School of Medicine, New York, NY, USA.
出版信息
Methods Mol Biol. 2023;2662:241-249. doi: 10.1007/978-1-0716-3167-6_21.
The development of single cell approaches has facilitated the investigation of cellular heterogeneity and cell type-specific gene expression in complex tissues. Adipose tissue depots contain lipid storing adipocytes as well as a diverse array of cell types that form the adipocyte niche and regulate adipose tissue function. Here, I describe two protocols for the isolation of single cells and nuclei from white and brown adipose tissue. Additionally, I provide a detailed workflow for isolation of cell type- or lineage-specific single nuclei using nuclear tagging and translating ribosome affinity purification (NuTRAP) mouse models.
单细胞方法的发展促进了对复杂组织中细胞异质性和细胞类型特异性基因表达的研究。脂肪组织包含储存脂质的脂肪细胞以及形成脂肪细胞龛并调节脂肪组织功能的多种细胞类型。在这里，我描述了从白色和棕色脂肪组织中分离单细胞和细胞核的两种方案。此外，我还提供了使用核标记和翻译核糖体亲和纯化（NuTRAP）小鼠模型分离细胞类型或谱系特异性单个核的详细工作流程。
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