G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Russia.
G.P. Somov Institute of Epidemiology and Microbiology, Rospotrebnadzor, 690087 Vladivostok, Russia.
Mar Drugs. 2023 Apr 13;21(4):238. doi: 10.3390/md21040238.
Nanoparticles formation is one of the ways to modulate the physicochemical properties and enhance the activity of original polysaccharides. For this purpose, based on the polysaccharide of red algae, κ-carrageenan (κ-CRG), it polyelectrolyte complex (PEC), with chitosan, were obtained. The complex formation was confirmed by ultracentrifugation in a Percoll gradient, with dynamic light scattering. According to electron microscopy and DLS, PEC is dense spherical particles with sizes in the range of 150-250 nm. A decrease in the polydispersity of the initial CRG was detected after the PEC formation. Simultaneous exposure of Vero cells with the studied compounds and herpes simplex virus type 1 (HSV-1) showed that the PEC exhibited significant antiviral activity, effectively inhibiting the early stages of virus-cell interaction. A two-fold increase in the antiherpetic activity (selective index) of PEC compared to κ-CRG was shown, which may be due to a change in the physicochemical characteristics of κ-CRG in PEC.
纳米颗粒的形成是调节物理化学性质和增强原始多糖活性的方法之一。为此，基于红藻多糖κ-卡拉胶（κ-CRG），与壳聚糖形成了聚电解质复合物（PEC）。通过在聚蔗糖梯度中超离心和动态光散射来确认复合物的形成。根据电子显微镜和 DLS，PEC 是致密的球形颗粒，粒径在 150-250nm 范围内。在 PEC 形成后，检测到初始 CRG 的多分散性降低。同时将研究化合物和单纯疱疹病毒 1（HSV-1）暴露于 Vero 细胞中，表明 PEC 表现出显著的抗病毒活性，有效地抑制了病毒与细胞相互作用的早期阶段。与 κ-CRG 相比，PEC 的抗疱疹活性（选择性指数）增加了一倍，这可能是由于 PEC 中 κ-CRG 的物理化学特性发生了变化。
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