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完成 TRB 家族：新鉴定的成员显示出古老的进化起源和独特的定位，但具有相似的相互作用。

Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions.

机构信息

Laboratory of Functional Genomics and Proteomics, Faculty of Science, National Centre for Biomolecular Research, Masaryk University, Brno, Czech Republic.

Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic.

出版信息

Plant Mol Biol. 2023 May;112(1-2):61-83. doi: 10.1007/s11103-023-01348-2. Epub 2023 Apr 28.

DOI:10.1007/s11103-023-01348-2
PMID:37118559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10167121/
Abstract

Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as associated with terminal telomeric repeats (telomeres) or short interstitial telomeric repeats in gene promoters (telo-boxes). They are also known to interact with several protein complexes: telomerase, Polycomb repressive complex 2 (PRC2) E(z) subunits and the PEAT complex (PWOs-EPCRs-ARIDs-TRBs). Here we characterize two novel members of the TRB family (TRB4 and TRB5). Our wide phylogenetic analyses have shown that TRB proteins evolved in the plant kingdom after the transition to a terrestrial habitat in Streptophyta, and consequently TRBs diversified in seed plants. TRB4-5 share common TRB motifs while differing in several others and seem to have an earlier phylogenetic origin than TRB1-3. Their common Myb-like domains bind long arrays of telomeric repeats in vitro, and we have determined the minimal recognition motif of all TRBs as one telo-box. Our data indicate that despite the distinct localization patterns of TRB1-3 and TRB4-5 in situ, all members of TRB family mutually interact and also bind to telomerase/PRC2/PEAT complexes. Additionally, we have detected novel interactions between TRB4-5 and EMF2 and VRN2, which are Su(z)12 subunits of PRC2.

摘要

端粒重复结合蛋白（TRB）属于一类具有 Myb 样结构域的蛋白家族，该结构域能够与端粒重复序列结合。拟南芥中有三个该家族的成员（TRB1、TRB2、TRB3）已被描述为与端粒末端重复序列（端粒）或基因启动子中的短间隔端粒重复序列（telobox）相关联。它们还与几个蛋白复合物相互作用：端粒酶、多梳抑制复合物 2（PRC2）E(z)亚基和 PEAT 复合物（PWOs-EPCRs-ARIDs-TRBs）。在这里，我们描述了 TRB 家族的两个新成员（TRB4 和 TRB5）。我们广泛的系统发育分析表明，TRB 蛋白在植物界进化，发生在陆地植物向陆地生活方式转变之后，因此在种子植物中 TRB 多样化。TRB4-5 具有共同的 TRB 基序，但在其他基序上存在差异，并且似乎具有比 TRB1-3 更早的进化起源。它们共同的 Myb 样结构域在体外能够结合长串的端粒重复序列，我们已经确定了所有 TRB 的最小识别基序为一个 telobox。我们的数据表明，尽管 TRB1-3 和 TRB4-5 在原位的定位模式明显不同，但 TRB 家族的所有成员都相互作用，并与端粒酶/PRC2/PEAT 复合物结合。此外，我们还检测到了 TRB4-5 与 EMF2 和 VRN2 的新相互作用，EMF2 和 VRN2 是 PRC2 的 Su(z)12 亚基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/d7220de916c8/11103_2023_1348_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/e69d48a64de1/11103_2023_1348_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/ea5b429fc320/11103_2023_1348_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/3b8e3e7fc1fc/11103_2023_1348_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/c8cdb210b5c4/11103_2023_1348_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/b1fb8fa4dd53/11103_2023_1348_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/667fa31bdb7f/11103_2023_1348_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/122cf15d2840/11103_2023_1348_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/d7220de916c8/11103_2023_1348_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/e69d48a64de1/11103_2023_1348_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/ea5b429fc320/11103_2023_1348_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/3b8e3e7fc1fc/11103_2023_1348_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/c8cdb210b5c4/11103_2023_1348_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/b1fb8fa4dd53/11103_2023_1348_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/667fa31bdb7f/11103_2023_1348_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/122cf15d2840/11103_2023_1348_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91f6/10167121/d7220de916c8/11103_2023_1348_Fig8_HTML.jpg

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