Department of Biomaterials, Faculty of Dentistry, Cairo University, Cairo, Egypt, Phone: +201015386763, e-mail:
Department of Biomaterials, Faculty of Dentistry, Cairo University, Cairo, Egypt.
J Contemp Dent Pract. 2022 Dec 1;23(12):1237-1244. doi: 10.5005/jp-journals-10024-3442.
The aim of this study was to evaluate the efficacy of grape seed extract (GSE) on remineralization of surface and subsurface enamel lesions compared to that of sodium fluoride (NaF).
A total of 20 intact bovine incisor crowns were separated from their roots and immersed in a demineralizing solution for 96 hours at 37°C to create artificial enamel lesions. The specimens were randomly divided into two groups ( = 10): 6.5% GSE solution and 1000 ppm NaF solution. The specimens were subjected to six daily pH cycles for 8 days. The microhardness test was carried out at three different stages: baseline, after artificial caries formation, and after pH cycling. Raman spectroscopy was used to evaluate the depth of enamel remineralization. Surface morphology and elemental analysis were assessed using a scanning electron microscope (SEM) and an energy dispersive X-ray (EDX) spectroscope, respectively. Statistical analysis was performed using SPSS 22.0 at a significance level of ≤ 0.05.
There was a significant increase in the mean values of enamel surface microhardness after pH cycles in the two groups compared to after artificial caries formation, but there was no significant difference between both groups. The B-type carbonate/phosphate (Ca/P) ratio at 10 and 40 µm depth revealed no significant difference between the two groups. Scanning electron microscope micrograph revealed occlusion of porosities and particle precipitation on the enamel surface of the two groups, while EDX results for the Ca/P ratio of the GSE and NaF groups were 1.59 and 1.60, respectively.
Grape seed extract and NaF are equally effective in remineralizing surface and subsurface artificial enamel lesions.
Grape seed extract can be considered a promising herbal material and a safe alternative to traditional NaF for the noninvasive treatment of enamel lesions.
本研究旨在评估葡萄籽提取物（GSE）在再矿化表面和亚表面釉质病损方面的疗效，与氟化钠（NaF）进行比较。
将 20 个完整的牛切牙冠从其根部分离出来，并在 37°C 的去矿化溶液中浸泡 96 小时，以形成人工釉质病损。标本随机分为两组（n=10）：6.5% GSE 溶液和 1000ppm NaF 溶液。标本进行了 6 次每日 pH 循环，持续 8 天。微硬度测试在三个不同阶段进行：基线、人工龋形成后和 pH 循环后。拉曼光谱用于评估釉质再矿化的深度。使用扫描电子显微镜（SEM）和能量色散 X 射线（EDX）光谱仪分别评估表面形态和元素分析。使用 SPSS 22.0 进行统计分析，显著性水平为 ≤ 0.05。
与人工龋形成后相比，两组在 pH 循环后釉质表面微硬度的平均值均显著增加，但两组之间无显著差异。在 10 和 40 µm 深度处的 B 型碳酸/磷酸盐（Ca/P）比值在两组之间无显著差异。扫描电子显微镜显示，两组釉质表面孔隙被封闭，颗粒沉淀，而 EDX 结果显示 GSE 和 NaF 组的 Ca/P 比值分别为 1.59 和 1.60。
葡萄籽提取物和 NaF 对表面和亚表面人工釉质病损的再矿化效果相同。
葡萄籽提取物可被视为一种有前途的草药材料，是传统 NaF 治疗釉质病损的安全替代方法。
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