溶液中DNA八聚体d（m5C-G-m5C-G-T-G-m5C-G）的发夹结构和双链形成：一项核磁共振研究。
Hairpin and duplex formation of the DNA octamer d(m5C-G-m5C-G-T-G-m5C-G) in solution. An NMR study.
作者信息
Orbons L P, van der Marel G A, van Boom J H, Altona C
出版信息
Nucleic Acids Res. 1986 May 27;14(10):4187-96. doi: 10.1093/nar/14.10.4187.
Abstract
The partly self-complementary DNA octamer d(m5C-G-m5C-G-T-G-m5C-G) was investigated by NMR spectroscopy in solution. It is demonstrated that this peculiar DNA fragment, under suitable conditions of concentration, salt and temperature, exclusively prefers to adopt a monomeric hairpin form with a stem of three Watson-Crick type base pairs and a loop of two residues. At high single strand concentration (8 mM DNA) and low temperature (i.e. below 295 K) the hairpin occurs in slow equilibrium with a B-dimer structure. At high ionic strength (greater than or equal to 100 mM Na+) and/or in the presence of methanol a third species appears, which is assigned to a Z-like dimer. In the B form, as well as in the Z dimer, the two central base pairs form G.T wobble pairs with the bases as major tautomers.
摘要
通过核磁共振光谱法对部分自我互补的DNA八聚体d（m5C-G-m5C-G-T-G-m5C-G）在溶液中进行了研究。结果表明，在合适的浓度、盐和温度条件下，这个特殊的DNA片段只倾向于形成单体发夹结构，其茎由三个沃森-克里克型碱基对组成，环由两个残基组成。在高单链浓度（8 mM DNA）和低温（即低于295 K）下，发夹结构与B-二聚体结构处于缓慢平衡状态。在高离子强度（大于或等于100 mM Na+）和/或存在甲醇的情况下，会出现第三种结构，被认定为Z-样二聚体。在B型结构以及Z二聚体中，两个中央碱基对形成G.T摆动对，碱基为主要互变异构体。
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