E2F2、MDM2和p16蛋白浓度与口腔鳞状细胞癌患者选定的社会人口学及临床病理特征之间的潜在关联

The Potential Association between E2F2, MDM2 and p16 Protein Concentration and Selected Sociodemographic and Clinicopathological Characteristics of Patients with Oral Squamous Cell Carcinoma.

作者信息

Świętek Agata, Gołąbek Karolina, Hudy Dorota, Gaździcka Jadwiga, Biernacki Krzysztof, Miśkiewicz-Orczyk Katarzyna, Zięba Natalia, Misiołek Maciej, Strzelczyk Joanna Katarzyna

机构信息

Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland.

Silesia LabMed Research and Implementation Centre, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland.

出版信息

Curr Issues Mol Biol. 2023 Apr 7;45(4):3268-3278. doi: 10.3390/cimb45040213.

DOI:10.3390/cimb45040213

PMID:37185737

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10137059/

Abstract

BACKGROUND

E2F transcription factor 2 (E2F2), murine double minute 2 (MDM2) and p16 are some of the key proteins associated with the control of the cell cycle. The aim of this study was to evaluate E2F2, MDM2 and p16 concentrations in the tumour and margin samples of oral squamous cell carcinoma and to assess their association with some selected sociodemographic and clinicopathological characteristics of the patients.

METHODS

The study group consisted of 73 patients. Protein concentrations were measured by enzyme-linked immunosorbent assay (ELISA).

RESULTS

There were no statistically significant differences in the levels of E2F2, MDM2 or p16 in the tumour samples as compared to the margin specimens. We found that patients with N0 showed significantly lower E2F2 concentrations than patients with N1 in the tumour samples and the median protein concentration of E2F2 was higher in HPV-negative patients in the tumour samples. Moreover, the level of p16 in the margin samples was lower in alcohol drinkers as compared to non-drinkers. Similar observations were found in concurrent drinkers and smokers compared to non-drinkers and non-smokers.

CONCLUSIONS

E2F2 could potentially promote tumour progression and metastasis. Moreover, our results showed a differential level of the analysed proteins in response to alcohol consumption and the HPV status.

摘要

背景

E2F转录因子2（E2F2）、鼠双微体2（MDM2）和p16是与细胞周期调控相关的一些关键蛋白。本研究的目的是评估口腔鳞状细胞癌肿瘤组织和切缘组织样本中E2F2、MDM2和p16的浓度，并评估它们与患者某些选定的社会人口学和临床病理特征之间的关联。

方法

研究组由73名患者组成。通过酶联免疫吸附测定（ELISA）测量蛋白质浓度。

结果

与切缘样本相比，肿瘤样本中E2F2、MDM2或p16的水平无统计学显著差异。我们发现，肿瘤样本中N0患者的E2F2浓度显著低于N1患者，且肿瘤样本中HPV阴性患者的E2F2蛋白浓度中位数更高。此外，饮酒者切缘样本中的p16水平低于不饮酒者。与不饮酒和不吸烟者相比，同时饮酒和吸烟的人也有类似的观察结果。