用于结直肠癌筛查的粪便中分离出的脱落结肠细胞的MiR-92a表达谱分析。
MiR-92a expression profiling of exfoliated colonocytes isolated from feces for colorectal cancer screening.
作者信息
Chen Yixin, He Wan, Chen Min, Zhao Lizhong, Shao Jianyong, Lin Shiyong, Li Baoqin, Xu Hualin, Li Ronghui, Feng Jun, Liang Ning, Meng Yue, Cheng Xiaojie, Zhang Weihua, Yu Haoyang, Xu Ruilian
机构信息
Department of Oncology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology) Shenzhen 518020, Guangdong, China.
Xiang'an Hospital of Xiamen University Xiamen 361102, Fujian, China.
出版信息
Am J Transl Res. 2023 Apr 15;15(4):2585-2597. eCollection 2023.
OBJECTIVE
This study seeks to assess the efficacy of exfoliated colonocytes isolated from feces (ECIF) miR-92a as a clinical colorectal cancer diagnostic marker in a larger cohort.
METHODS
Clinicopathologic data from colorectal cancer patients and health controls that underwent colonoscopy, as well as patients of other cancers diagnosed, were included. A total of 963 Chinese participants were enrolled, with 292 (27.4%) having colorectal cancer, 140 (14.5%) having other types of cancer, e.g., pancreatic, liver, oral, bile duct, esophagus, and stomach cancer, 171 (17.8%) having infection in the intestine, rectal, stomach, appendix, and gastrointestinal ulcer, and 360 (37.4%) of healthy controls. ECIF samples were gathered and miR-92a levels were detected using TaqMan probe-based miR-92a real-time quantitative PCR (RT-qPCR) kit developed by Shenzhen GeneBioHealth Co., Ltd.
RESULTS
Through a series of experiments, we demonstrated that the Ep-LMB/Vi-LMB magnetic separation system is feasible, highly specific, and highly sensitive at a cutoff value of 1053 copies per 6 ng of ECIF RNA. ECIF miR-92a levels were significantly higher in colorectal cancer patients than in controls. Colorectal cancer detection sensitivity and specificity were 87.3% and 86.9% respectively. Furthermore, the performance of this miR-92a detection kit demonstrated that it is an effective tool for colorectal cancer, with a high sensitivity of 84.1%, even in early cancer stages (0, I, and II). Furthermore, tumor removal resulted in lower stool miR-92a levels (3.21±0.58 vs. 2.14±1.14, P < 0.0001, n = 65).
CONCLUSION
Finally, the miR-92a RT-qPCR kit detects ECIF-increased miR-92a and could be used for colorectal cancer screening.
目的
本研究旨在评估从粪便中分离的脱落结肠细胞（ECIF）中的miR-92a作为临床结直肠癌诊断标志物在更大队列中的有效性。
方法
纳入接受结肠镜检查的结直肠癌患者和健康对照以及其他癌症确诊患者的临床病理数据。共招募了963名中国参与者，其中292名（27.4%）患有结直肠癌，140名（14.5%）患有其他类型癌症，如胰腺癌、肝癌、口腔癌、胆管癌、食管癌和胃癌，171名（17.8%）患有肠道、直肠、胃、阑尾和胃肠道溃疡感染，360名（37.4%）为健康对照。收集ECIF样本，并使用深圳基因生物健康有限公司开发的基于TaqMan探针的miR-92a实时定量PCR（RT-qPCR）试剂盒检测miR-92a水平。
结果
通过一系列实验，我们证明Ep-LMB/Vi-LMB磁分离系统是可行的，具有高度特异性，在每6 ng ECIF RNA 1053拷贝的临界值下具有高度敏感性。结直肠癌患者的ECIF miR-92a水平显著高于对照组。结直肠癌检测的敏感性和特异性分别为87.3%和86.9%。此外，该miR-92a检测试剂盒的性能表明，它是结直肠癌的有效检测工具，即使在癌症早期阶段（0期、I期和II期）也具有84.1%的高敏感性。此外，肿瘤切除导致粪便中miR-92a水平降低（3.21±0.58对2.14±1.14，P < 0.0001，n = 65）。
结论
最后，miR-92a RT-qPCR试剂盒可检测到ECIF中升高的miR-92a，可用于结直肠癌筛查。
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