体外研究氟化亚锡- CPP-ACP 复合体的抗蚀能力。

Erosion-inhibiting potential of the stannous fluoride-enriched CPP-ACP complex in vitro.

机构信息

Adelaide Dental School, Level 10, Adelaide Health and Medical Sciences (AHMS) Building, University of Adelaide, Cnr George St and North Tce, Adelaide, SA, 5005, Australia.

Future Industries Institute, University of South Australia, Mawson Lakes, Australia.

出版信息

Sci Rep. 2023 May 16;13(1):7940. doi: 10.1038/s41598-023-34884-4.

DOI:10.1038/s41598-023-34884-4

PMID:37193788

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10188480/

Abstract

Currently available anti-erosive agents only provide partial protection, emphasizing the need to enhance their performance. By characterizing erosive enamel wear at the nanoscale, the aim of this in vitro study was to assess the anti-erosive effects of SnF and CPP-ACP both individually and synergistically. Erosion depths were assessed longitudinally on 40 polished human enamel specimens after 1, 5, and 10 erosion cycles. Each cycle comprised one-min erosion in citric acid (pH 3.0) and one-min treatment in whole saliva (control group) or a slurry of one of the three anti-erosive pastes (10% CPP-ACP; 0.45% SnF (1100 ppm F); or SnF/CPP-ACP (10% CPP-ACP + 0.45% SnF)) (n = 10 per group). Scratch depths were assessed longitudinally in separate experiments using a similar protocol after 1, 5, and 10 cycles. Compared with the control groups, all slurries reduced erosion depths after 1 cycle (p ≤ 0.004) and scratch depths after 5 cycles (p ≤ 0.012). The order of anti-erosive potential was SnF/CPP-ACP > SnF > CPP-ACP > control for erosion depth analysis, and SnF/CPP-ACP > (SnF = CPP-ACP) > control for scratch depth analysis. These data provide 'proof of concept' evidence that SnF/CPP-ACP has superior anti-erosive potential compared to SnF or CPP-ACP alone.

摘要

目前可用的抗蚀剂仅提供部分保护，强调需要增强其性能。通过在纳米尺度上对腐蚀性牙釉质磨损进行特征描述，本体外研究旨在评估 SnF 和 CPP-ACP 单独和协同使用的抗蚀效果。在 40 个抛光的人牙釉质标本上进行了纵向评估，在 1、5 和 10 个侵蚀循环后评估了侵蚀深度。每个循环包括在柠檬酸（pH 3.0）中进行一分钟的侵蚀和在全唾液中进行一分钟的处理（对照组）或三种抗蚀糊剂之一的处理（10% CPP-ACP；0.45% SnF（1100 ppm F）；或 SnF/CPP-ACP（10% CPP-ACP+0.45% SnF））（每组 10 个标本）。使用类似的方案，在单独的实验中在 1、5 和 10 个循环后进行了纵向划痕深度评估。与对照组相比，所有糊剂在第 1 个循环后均降低了侵蚀深度（p≤0.004），在第 5 个循环后降低了划痕深度（p≤0.012）。抗蚀潜力的顺序为：对于侵蚀深度分析，SnF/CPP-ACP＞SnF＞CPP-ACP＞对照组；对于划痕深度分析，SnF/CPP-ACP＞（SnF＝CPP-ACP）＞对照组。这些数据提供了“概念验证”证据，表明 SnF/CPP-ACP 与单独使用 SnF 或 CPP-ACP 相比具有更强的抗蚀潜力。