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:首个完整叶绿体基因组的结构与比较分析。

The First Complete Chloroplast Genome of : Structure and Comparative Analysis.

机构信息

Department of Biology, College of Science, University of Jeddah, Jeddah 21493, Saudi Arabia.

Department of Biological Sciences, Faculty of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia.

出版信息

Genes (Basel). 2023 Apr 26;14(5):976. doi: 10.3390/genes14050976.

Abstract

is a member of the Boraginaceae family. This plant is widely distributed in tropical regions and has a great deal of medical value as well as economic importance. In the current study, the complete chloroplast (cp) genome of was sequenced, assembled, annotated, and reported. This circular chloroplast genome had a size of 148,711 bp, with a quadripartite structure alternating between a pair of repeated inverted regions (26,897-26,901 bp) and a single copy region (77,893 bp). Among the 134 genes encoded by the cp genome, there were 89 protein-coding genes, 37 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. A total of 1387 tandem repeats were detected, with the hexanucleotides class making up 28 percent of the repeats. has 26,303 codons in its protein-coding regions, and leucine amino acid was the most frequently encoded amino acid in contrast to cysteine. In addition, 12 of the 89 protein-coding genes were found to be under positive selection. The phyloplastomic taxonomical clustering of the Boraginaceae species provides further evidence that chloroplast genome data are reliable not only at family level but also in deciphering the phylogeny at genus level (e.g., ).

摘要

是紫草科的一员。这种植物广泛分布于热带地区,具有很大的药用价值和经济重要性。在本研究中,我们对 的完整叶绿体(cp)基因组进行了测序、组装、注释和报告。这个圆形叶绿体基因组大小为 148711bp,具有由一对重复的反向区域(26897-26901bp)和一个单一拷贝区域(77893bp)交替组成的四分体结构。在 cp 基因组编码的 134 个基因中,有 89 个蛋白编码基因、37 个转移 RNA(tRNA)基因和 8 个核糖体 RNA(rRNA)基因。总共检测到 1387 个串联重复,六核苷酸类占重复的 28%。在其蛋白编码区有 26303 个密码子,亮氨酸是最常编码的氨基酸,而半胱氨酸则相反。此外,在 89 个蛋白编码基因中有 12 个被发现受到正选择。紫草科植物的叶绿体基因组系统发育分类聚类进一步证明,叶绿体基因组数据不仅在科水平上可靠,而且在解析属水平的系统发育(例如 )方面也可靠。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd81/10217729/7b78440ea46d/genes-14-00976-g001.jpg

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