推进分子印迹聚合物在生物分析中的应用：选择性提取人尿液中的可替宁。
Advancing the use of molecularly imprinted polymers in bioanalysis: the selective extraction of cotinine in human urine.
机构信息
Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University, Richmond, VA 23298, USA.
Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284, USA.
出版信息
Bioanalysis. 2023 Apr;15(8):465-477. doi: 10.4155/bio-2023-0019. Epub 2023 May 31.
To characterize a molecularly imprinted polymer via precipitation polymerization for the extraction of cotinine in urine. The polymer was created via precipitation polymerization. Physical characteristics of the polymer were assessed via scanning electron microscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis. The polymer adsorption capacity was assessed and an solid-phase extraction method from urine by LC-MS/MS was developed. The polymer had small, spherical morphology and little thermal decomposition. The extraction method yielded cotinine recoveries of 77-103% in urine. The molecularly imprinted polymer adsorption capacity for cotinine was 448.2 ± 2.1 μg/mg. Common interferants did not affect cotinine's extraction. The resulting polymer was determined to be specific for cotinine and can be used for the detection of cotinine in urine for clinical samples.
通过沉淀聚合来对可替宁进行印迹，以提取尿液中的可替宁。该聚合物是通过沉淀聚合制备的。通过扫描电子显微镜、傅里叶变换红外光谱和热重分析评估了聚合物的物理特性。评估了聚合物的吸附能力，并开发了一种通过 LC-MS/MS 从尿液中进行固相萃取的方法。该聚合物具有较小的球形形态，热分解很少。该萃取方法在尿液中的可替宁回收率为 77-103%。分子印迹聚合物对可替宁的吸附容量为 448.2±2.1μg/mg。常见的干扰物质不会影响可替宁的提取。结果表明，该聚合物对可替宁具有特异性，可用于检测临床样本中的尿液中的可替宁。
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