乙型肝炎病毒相关肝细胞癌中 5-羟甲基胞嘧啶的减少：一项横断面研究。
Decrease of 5-hydroxymethylcytosine in hepatitis B virus-related hepatocellular carcinoma: A cross-sectional study.
机构信息
Division of Liver Diseases, The Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China.
Department of Pathology, the Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei, China.
出版信息
Medicine (Baltimore). 2023 Jun 2;102(22):e33943. doi: 10.1097/MD.0000000000033943.
Many epigenetic studies had found the decrease of 5-hydroxymethylcytosine (5-hmC) in various tumor tissues. However, limited information is available for hepatitis B virus-related hepatocellular carcinoma (HBV-related HCC). The present study aimd to investigate whether the decrease also existed in tumor tissues of HBV-related HCC and, if possible, to disclose its mechanism. We used immunohistochemistry and Image Pro Plus 6.0 Image Analysis Software to quantify the expression of 5-hmC, 5-methylcytosine, 10-eleven translocation (TET), isocitrate dehydrogenase (IDH) in pathological sections of tumor tissues and its para cancerous tissues of 40 HBV-related HCC patients. Our results showed that 5-hmC was decreased while 5-methylcytosine was increased in tumor tissues. We also detected TET1 and IDH2 were decreased in the tumor tissues and the decrease were positively correlated with the 5-hmC. The results suggested that the deficiency of 5-hmC was an epigenetic characteristic of HBV-related HCC and was mainly caused by the decrease of TET1 and IDH2.
许多表观遗传学研究发现，在各种肿瘤组织中 5-羟甲基胞嘧啶（5-hmC）的含量减少。然而，关于乙型肝炎病毒相关肝细胞癌（HBV 相关 HCC）的信息有限。本研究旨在探讨这种减少是否也存在于 HBV 相关 HCC 的肿瘤组织中，如果可能的话，揭示其机制。我们使用免疫组织化学和 Image Pro Plus 6.0 图像分析软件，对 40 例 HBV 相关 HCC 患者肿瘤组织及其癌旁组织的病理切片中 5-hmC、5-甲基胞嘧啶、10-十一烯转移酶（TET）、异柠檬酸脱氢酶（IDH）的表达进行了定量分析。我们的结果表明，肿瘤组织中 5-hmC 减少，而 5-甲基胞嘧啶增加。我们还检测到肿瘤组织中 TET1 和 IDH2 减少，并且这种减少与 5-hmC 呈正相关。结果表明，5-hmC 的缺乏是 HBV 相关 HCC 的一种表观遗传特征，主要是由 TET1 和 IDH2 的减少引起的。
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