Nanoencapsulation of garlic essential oil using chitosan nanopolymer and its antifungal and anti-aflatoxin B1 efficacy in vitro and in situ.

The present study investigated the comparative efficacy of garlic essential oil (GEO) and its nanoencapsulated within chitosan nanomatrix (GEO-CSNPs) as a novel preservative for the protection of stored food commodities from fungal infestations, aflatoxin B1 (AFB1) contamination and lipid peroxidation against a toxigenic strain of Aspergillus flavus. GC-MS examination of GEO showed the presence of allyl methyl tri-sulfide (23.10 %) and diallyl sulfide (19.47 %) as the major components. GEO-CSNPs were characterized through TEM micrograph, DLS, XRD, and FTIR instrumentation. During the in-vitro investigation, GEO-CSNPs at 1.0 μL/mL dose completely inhibited the growth of A. flavus while preventing the synthesis of AFB at 0.75 μL/mL compared to the pure GEO. The biochemical analysis reveals that A. flavus exposed to GEO-CSNPs significantly changed its ergosterol level, ions leakage, mitochondrial membrane potential (MMP), and antioxidant system. Additionally, GEO-CSNPs exhibited enhanced antioxidant activity against DPPH compared with GEO. Likewise, during in-situ experiments on A. hypogea GEO-CSNPs MIC and 2 MIC concentration prohibited fungal development, AFB synthesis, and lipid peroxidation or inflicting any negative impacts on germinating seeds. Overall, investigations concluded that GEO-CSNPs could be used as a novel preservative agent to improve the shelf life of stored food commodities.