Samadian Hadi, Vahidi Reza, Salehi Majid, Hosseini-Nave Hossein, Shahabi Arman, Zanganeh Saeed, Lashkari Mahla, Kouhbananinejad Seyedeh Mehrnaz, Rezaei Kolarijani Nariman, Amini Seyed Mohammad, Asadi-Shekari Majid, Mirzaei Parsa Mohamad Javad
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Basic Med Sci. 2023;26(6):708-716. doi: 10.22038/IJBMS.2023.68897.15016.
The main objective of the current assay was to evaluate the antibacterial and regenerative effects of hydrogel nanocomposite containing pure natural zeolite (clinoptilolite) integrated with alginate (Alg) as wound healing/dressing biomaterials.
The zeolites were size excluded, characterized by SEM, DLS, XRD, FTIR, and XRF, and then integrated into Alg hydrogel followed by calcium chloride crosslinking. The Alg and alginate zeolite (Alg/Zeo) hydrogel was characterized by swelling and weight loss tests, also the antibacterial, hemocompatibility, and cell viability tests were performed. In animal studies, the burn wound was induced on the back of rats and treated with the following groups: control, Alg hydrogel, and Alg/Zeo hydrogel.
The results showed that the hydrodynamic diameter of zeolites was 367 ± 0.2 nm. Zeolites did not show any significant antibacterial effect, however, the hydrogel nanocomposite containing zeolite had proper swelling as well as hemocompatibility and no cytotoxicity was observed. Following the creation of a third-degree burn wound on the back of rats, the results indicated that the Alg hydrogel and Alg/Zeo nanocomposite accelerated the wound healing process compared with the control group. Re-epithelialization, granulation tissue thickness, collagenization, inflammatory cell recruitment, and angiogenesis level were not significantly different between Alg and Alg/Zeo nanocomposite.
These findings revealed that although the incorporation of zeolites did not induce a significant beneficial effect in comparison with Alg hydrogel, using zeolite capacity in hydrogel for loading the antibiotics or other effective compounds can be considered a promising wound dressing.
本实验的主要目的是评估含有纯天然沸石（斜发沸石）与藻酸盐（Alg）整合的水凝胶纳米复合材料作为伤口愈合/敷料生物材料的抗菌和再生效果。
对沸石进行尺寸排阻，通过扫描电子显微镜（SEM）、动态光散射（DLS）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和X射线荧光光谱（XRF）进行表征，然后将其整合到Alg水凝胶中，接着用氯化钙进行交联。通过溶胀和失重测试对Alg和藻酸盐沸石（Alg/Zeo）水凝胶进行表征，同时进行抗菌、血液相容性和细胞活力测试。在动物研究中，在大鼠背部诱导烧伤创面，并分为以下几组进行治疗：对照组、Alg水凝胶组和Alg/Zeo水凝胶组。
结果表明，沸石的流体动力学直径为367±0.2纳米。沸石未显示出任何显著的抗菌效果，然而，含沸石的水凝胶纳米复合材料具有适当的溶胀性以及血液相容性，且未观察到细胞毒性。在大鼠背部造成三度烧伤创面后，结果表明，与对照组相比，Alg水凝胶和Alg/Zeo纳米复合材料加速了伤口愈合过程。Alg和Alg/Zeo纳米复合材料之间的再上皮化、肉芽组织厚度、胶原化、炎症细胞募集和血管生成水平无显著差异。
这些发现表明，尽管与Alg水凝胶相比，加入沸石并未产生显著的有益效果，但利用水凝胶中沸石负载抗生素或其他有效化合物的能力可被视为一种有前景的伤口敷料。
