甘油分离的有丝分裂器中染色体运动的诱导：核苷酸特异性以及抗动力蛋白和肌球蛋白血清对运动的影响。

INDUCTION OF CHROMOSOME MOTION IN THE GLYCEROL-ISOLATED MITOTIC APPARATUS: NUCLEOTIDE SPECIFICITY AND EFFECTS OF ANTIDYNEIN AND MYOSIN SERA ON THE MOTION.

作者信息

Sakai Hikoichi, Mabuchi Issei, Shimoda Shumei, Kuriyama Ryoko, Ogawa Kazuo, Mohri Hideo

机构信息

Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Bunkyo-ku.

Department of Biology, College of General Education, University of Tokyo, Meguro-ku.

出版信息

Dev Growth Differ. 1976;18(3):211-219. doi: 10.1111/j.1440-169X.1976.00211.x.

DOI:10.1111/j.1440-169X.1976.00211.x

PMID:37282068

Abstract

Chromosome motion in glycerol-isolated mitotic apparatus (MA) of sea urchin and starfish eggs was investigated with respect to nucleotide specificity and the effects of antisera against tryptic fragment (Fragment A) of flagellar dynein and starfish egg myosin. The motion was highly specific for ATP. GTP, ITP, CTP, UTP, and ADP caused no displacement of the chromosomes towards the poles. The anti-Fragment A serum completely inhibited chromosome motion in the MA of the sea urchin egg, while antiserum against starfish egg myosin as well as its γ-globulin fraction did not inhibit the motion in the isolated MA of the starfish egg, suggesting that chromosome motion depends upon dynein-microtubule but not upon myosin-actin interaction. In addition, colchicine completely suppressed the chromosome motion in vitro.

摘要

针对核苷酸特异性以及抗鞭毛动力蛋白胰蛋白酶片段（片段A）和海星卵肌球蛋白抗血清的影响，对海胆和海星卵甘油分离有丝分裂器（MA）中的染色体运动进行了研究。该运动对ATP具有高度特异性。GTP、ITP、CTP、UTP和ADP均未使染色体向两极移动。抗片段A血清完全抑制了海胆卵MA中的染色体运动，而抗海星卵肌球蛋白血清及其γ球蛋白部分并未抑制海星卵分离MA中的运动，这表明染色体运动取决于动力蛋白-微管相互作用，而非肌球蛋白-肌动蛋白相互作用。此外，秋水仙碱在体外完全抑制了染色体运动。