Zhang Ya-Ping, Tian Lei, Xie Xiao-Qian, Wang Ya-Ting, Lyu Peng, Xi Zhu-Ge
School of Public Health and Management,Binzhou Medical University, Yantai 264000.
Institute of Environmental and Operational Medicine, Academy of Military Medicine, Academy of Military Sciences, Tianjin 300050, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022 Nov;38(6):760-765. doi: 10.12047/j.cjap.6379.2022.138.
To investigate the effects of polystyrene nanoplastics (PS-NPs) exposure during gestation on the growth and neurotoxicity of fetal rats. Twenty-seven SD pregnant rats were randomly divided into 9 groups with three rats in each group. The experimental group of PS-NPs was given 0.5, 2.5, 10 and 50 mg/kg of PS-NPs suspension with different particle sizes (25 and 50 nm) by gavage, wihe the control group was given ultrapure water by gavage. The time of gavage is from the 1st to the 18th day of pregnancy. The morphological changes of the placenta were observed; compare the number of male and female fetuses, live/dead/absorbed fetuses, body weight, body length, placental weight, and organ coefficients of kidney, liver, brain and intestine of fetal rats; the prefrontal cortex, hippocampus and striatum of the fetal rats were taken to measure related biochemical indicators. Compared with the control group, the placenta of the PS-NPs exposed group was found to have structural damage, which increased in a dose-dependent manner. The area ratio of trophoblast was significantly increased (＜0.05), and the area ratio of labyrinth was significantly decreased (＜0.05); In the prefrontal cortex, hippocampus and striatum of fetal rats, the levels of IL-1β, IL -6 and TNF-α were significantly increased in the 10 and 50 mg/kg PS-NPs exposed group (＜0.05), and more significantly elevated in the 25 nm group than those in the 50 nm group at 10 mg/kg exposure (＜0.05) the CAT activity was significantly decreased in 2.5, 10 and 50 mg/kg PS-NPs exposure groups (＜0.05), while the SOD and GSH-Px activities were significantly decreased in 25 nm exposure groups and 2.5, 10 and 50 mg/kg 50 nm PS-NPs exposure groups (＜0.05), the MDA content was significantly increased in 10, 50 mg/kg 25 nm PS-NPs exposure groups and 50 mg/kg 50 nm PS-NPs exposure groups (＜0.05). Maternal PS-NPs exposure during gestation may affect the growth and development of fetal rats by damaging the placental barrier and produce neurotoxicity in fetal rats, causing oxidative stress and inflammatory responses in various brain regions, and smaller particle sizes and higher doses of polystyrene nanoplastic exposure have more significant neurotoxic effects on the offspring.
为研究孕期暴露于聚苯乙烯纳米塑料（PS-NPs）对胎鼠生长及神经毒性的影响。将27只SD孕鼠随机分为9组，每组3只。PS-NPs实验组分别经口灌胃给予0.5、2.5、10和50 mg/kg不同粒径（25和50 nm）的PS-NPs悬浮液，对照组经口灌胃给予超纯水。灌胃时间为妊娠第1天至第18天。观察胎盘的形态学变化；比较胎鼠的雌雄数量、活/死/吸收胎鼠数量、体重、体长、胎盘重量以及肾、肝、脑和肠的脏器系数；取胎鼠前额叶皮质、海马和纹状体测定相关生化指标。与对照组相比，PS-NPs暴露组胎盘出现结构损伤，且呈剂量依赖性增加。滋养层面积比显著增加（＜0.05），迷路面积比显著降低（＜0.05）；在胎鼠前额叶皮质、海马和纹状体中，10和50 mg/kg PS-NPs暴露组的IL-1β、IL -6和TNF-α水平显著升高（＜0.05），在10 mg/kg暴露时，25 nm组比50 nm组升高更显著（＜0.05）；2.5、10和50 mg/kg PS-NPs暴露组的CAT活性显著降低（＜0.05），而25 nm暴露组以及2.5、10和50 mg/kg 50 nm PS-NPs暴露组的SOD和GSH-Px活性显著降低（＜0.05），10、50 mg/kg 25 nm PS-NPs暴露组和50 mg/kg 50 nm PS-NPs暴露组的MDA含量显著增加（＜0.05）。孕期母体暴露于PS-NPs可能通过损伤胎盘屏障影响胎鼠的生长发育，并对胎鼠产生神经毒性，导致各脑区氧化应激和炎症反应，且更小粒径和更高剂量的聚苯乙烯纳米塑料暴露对后代的神经毒性作用更显著。
