食蟹猴疟原虫复合体环子孢子蛋白基因的进化概况

Evolutionary profile of the circumsporozoite gene of the Plasmodium cynomolgi complex.

作者信息

Enea V, Galinski M, Schmidt E, Gwadz R, Nussenzweig R S

出版信息

J Mol Biol. 1986 Apr 20;188(4):721-6. doi: 10.1016/s0022-2836(86)80017-1.

Abstract

The circumsporozoite genes and flanking sequences of the Ceylon, Gombak, London, NIH and Mulligan strains of the Plasmodium cynomolgi complex were isolated by molecular cloning and compared. About 11,000 bases of the Gombak clone were mapped in detail and found to have their exact counterparts in all the other strains. In contrast the epitope-encoding region, a 600-base sequence consisting of short tandem repeats, exhibited no homology with any of the other clones. These findings show that different regions of the circumsporozoite gene evolve in sharply different modes.

摘要

通过分子克隆分离并比较了食蟹猴疟原虫复合体的锡兰、贡巴克、伦敦、美国国立卫生研究院和穆利根株的环子孢子基因及其侧翼序列。详细绘制了贡巴克克隆的约11,000个碱基，并发现它们在所有其他菌株中都有确切的对应序列。相比之下，由短串联重复序列组成的600个碱基的表位编码区与任何其他克隆均无同源性。这些发现表明，环子孢子基因的不同区域以截然不同的方式进化。