银纳米颗粒对有或无牙周病患者口腔生物膜的杀菌活性
Bactericidal Activity of Silver Nanoparticles on Oral Biofilms Related to Patients with and without Periodontal Disease.
作者信息
Hernández-Venegas Perla Alejandra, Martínez-Martínez Rita Elizabeth, Zaragoza-Contreras Erasto Armando, Domínguez-Pérez Rubén Abraham, Reyes-López Simón Yobanny, Donohue-Cornejo Alejandro, Cuevas-González Juan Carlos, Molina-Frechero Nelly, Espinosa-Cristóbal León Francisco
机构信息
Chemical Biological Department, Institute of Biomedical Sciences, Autonomous University of Juarez City (UACJ), Envolvente del PRONAF and Estocolmo s/n, Ciudad Juárez 32310, Chihuahua, Mexico.
Master Program in Advanced Dentistry, Faculty of Dentistry, Autonomous University of San Luis Potosi, Manuel Nava Avenue, Universitary Campus, San Luis Potosí 78290, San Luis Potosi, Mexico.
出版信息
J Funct Biomater. 2023 Jun 2;14(6):311. doi: 10.3390/jfb14060311.
BACKGROUND AND OBJECTIVES
Periodontal disease (PD) is a multifactorial oral disease regularly caused by bacterial biofilms. Silver nanoparticles (AgNP) have offered good antimicrobial activity; moreover, there is no available scientific information related to their antimicrobial effects in biofilms from patients with PD. This study reports the bactericidal activity of AgNP against oral biofilms related to PD.
MATERIALS AND METHODS
AgNP of two average particle sizes were prepared and characterized. Sixty biofilms were collected from patients with (30 subjects) and without PD (30 subjects). Minimal inhibitory concentrations of AgNP were calculated and the distribution of bacterial species was defined by polymerase chain reaction.
RESULTS
Well-dispersed sizes of AgNP were obtained (5.4 ± 1.3 and 17.5 ± 3.4 nm) with an adequate electrical stability (-38.2 ± 5.8 and -32.6 ± 5.4 mV, respectively). AgNP showed antimicrobial activities for all oral samples; however, the smaller AgNP had significantly the most increased bactericidal effects (71.7 ± 39.1 µg/mL). The most resistant bacteria were found in biofilms from PD subjects ( < 0.05). and . were present in all PD biofilms (100%).
CONCLUSIONS
The AgNP showed efficient bactericidal properties as an alternative therapy for the control or progression of PD.
背景与目的
牙周病（PD）是一种常由细菌生物膜引起的多因素口腔疾病。银纳米颗粒（AgNP）具有良好的抗菌活性；此外，尚无关于其对牙周病患者生物膜抗菌作用的科学信息。本研究报告了AgNP对与牙周病相关的口腔生物膜的杀菌活性。
材料与方法
制备并表征了两种平均粒径的AgNP。从患有牙周病（30名受试者）和未患牙周病（30名受试者）的患者中收集了60个生物膜。计算了AgNP的最低抑菌浓度，并通过聚合酶链反应确定了细菌种类的分布。
结果
获得了分散良好的AgNP粒径（分别为5.4±1.3和17.5±3.4 nm），具有足够的电稳定性（分别为-38.2±5.8和-32.6±5.4 mV）。AgNP对所有口腔样本均显示出抗菌活性；然而，较小的AgNP具有显著更高的杀菌效果（71.7±39.1 µg/mL）。在牙周病受试者的生物膜中发现了最具抗性的细菌（<0.05）。 和 在所有牙周病生物膜中均存在（100%）。
结论
AgNP作为控制或延缓牙周病进展的替代疗法显示出有效的杀菌特性。
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