Universidade Estadual Paulista (UNESP), Faculdade de Odontologia, Departamento de Odontologia Preventiva e Restauradora, Araçatuba, São Paulo, Brasil.
Universidade do Oeste Paulista (UNOESTE), Programa de Pós-graduação em Ciências da Saúde, Presidente Prudente, São Paulo, Brasil.
J Appl Oral Sci. 2023 Jun 7;31:e20230155. doi: 10.1590/1678-7757-2023-0115. eCollection 2023.
To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions.
Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely- (calcium fluoride; CaF2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF2, FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log10-transformed) were subjected to ANOVA and the Student-Newman-Keuls' test (p<0.05).
We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid.
Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions.
评估氟化物（F）凝胶补充微米或纳米级三聚磷酸钠（TMPmicro 和 TMPnano）对龋样病变体外再矿化的影响。
根据表面硬度（SH）选择牛牙釉质表面下病变（n=168），并随机分为 7 组（n=24/组）：空白对照（无 F/TMP）、4500ppm F（4500F）、4500F+2.5%TMPnano（2.5%Nano）、4500F+5%TMPnano（5% Nano）、4500F+5%TMPmicro（5% Micro）、9000ppm F（9000F）和 12300ppm F（酸性凝胶）。凝胶以薄层形式涂敷一分钟。一半的样本块进行六天的 pH 循环，而其余样本用于松散结合（氟化钙；CaF2）和牢固结合（氟磷灰石；FA）氟化物分析。表面硬度恢复百分比（%SHR）、釉下病变面积（ΔKHN）、釉质中的 CaF2、FA、钙（Ca）和磷（P）的百分比被测定。数据（经对数转换）进行方差分析和 Student-Newman-Keuls 检验（p<0.05）。
我们观察到不含 TMP 的 F 浓度与%SHR 和ΔKHN 之间存在剂量反应关系。2.5%Nano 和 5% Micro 与 9000F 和酸性凝胶相比达到了相似的%SHR。对于ΔKHN，空白对照和 5%Nano 凝胶具有最高值，而 5%Micro、2.5%Nano、9000F 和酸性凝胶具有最低值。除空白对照和酸性凝胶外，所有组的 CaF2 保留值相似。我们发现纳米级 TMP 组的 Ca 浓度增加。关于 P，TMP 组的形成和保留与 9000F 和酸性凝胶相似。
在低氟凝胶中添加 2.5%纳米级或 5%微米级 TMP 可增强人工龋样病变的体外再矿化。
