Fonterra Co-operative Group, Ltd, Laboratory Operations, PO Box 7, Waitoa 3341, New Zealand.
J AOAC Int. 2023 Sep 1;106(5):1230-1236. doi: 10.1093/jaoacint/qsad079.
Taurine is recognized as an essential growth factor and as being critical in the maintenance of functional tissue regulation.
To evaluate the analytical performance of a hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method for compliance with AOAC Standard Method Performance Requirements (SMPR®) for taurine analysis described in SMPR 2014.013.
Following protein precipitation with Carrez solutions, taurine is extracted and separated by HILIC with detection by triple quadrupole MS using multiple reaction monitoring (MRM). Stable isotope labeled (SIL) taurine internal standard is used for quantification to correct for losses in extraction and variations in ionization in the ion source.
The method was shown to meet the requirements specified in the SMPR with a linear range of 0.27-2700 mg/hg RTF (ready-to-feed), a limit of detection of 0.14 mg/hg RTF, acceptable recovery of 97.2-100.1%, and acceptable repeatability of 1.6-6.4% relative standard deviation. Additionally, the method was found to have no statistically significant bias compared with reference values for National Institute of Standards and Technology (NIST) 1849a certified reference material (CRM) (P-value = 0.95) and 1869 CRM (P-value = 0.31), and with results from AOAC 997.05 (P-value = 0.10).
A recent review of the method and validation data by the Stakeholder Program on Infant Formula and Adult Nutritionals (SPIFAN) Expert Review Panel (ERP) found that this method met all the criteria for analysis of taurine specified in SMPR 2014.013 and voted to adopt this method as First Action AOAC Official MethodSM2022.03.
A method for the analysis of taurine in infant formulas and adult nutritionals by HILIC-MS/MS is described. A single-laboratory validation (SLV) study demonstrated the applicability of the method to meet requirements of SMPR 2014.013. In December 2022, the SPIFAN ERP voted to adopt this method as First Action AOAC Official Method 2022.03.
牛磺酸被认为是一种必需的生长因子，对维持功能组织调节至关重要。
评估亲水相互作用液相色谱-串联质谱（HILIC-MS/MS）方法的分析性能，以符合 AOAC 标准方法性能要求（SMPR®）中描述的 2014.013 年牛磺酸分析 SMPR。
用 Carrez 溶液进行蛋白质沉淀后，牛磺酸通过 HILIC 提取和分离，并用三重四极杆 MS 通过多重反应监测（MRM）进行检测。使用稳定同位素标记（SIL）牛磺酸内标进行定量，以校正提取过程中的损失和离子源中离子化的变化。
该方法被证明符合 SMPR 中规定的要求，具有 0.27-2700mg/hg RTF（即食）的线性范围、0.14mg/hg RTF 的检测限、97.2-100.1%的可接受回收率和 1.6-6.4%的可接受重复性相对标准偏差。此外，与美国国家标准与技术研究院（NIST）1849a 认证参考物质（CRM）（P 值=0.95）和 1869 CRM（P 值=0.31）的参考值相比，该方法无统计学意义上的偏差，与 AOAC 997.05 的结果相比（P 值=0.10）也是如此。
婴儿配方食品和成人营养物质利益相关者计划（SPIFAN）专家审查小组（ERP）最近对该方法和验证数据进行了审查，发现该方法符合 SMPR 2014.013 中规定的牛磺酸分析的所有标准，并投票通过该方法作为第一行动 AOAC 官方方法 2022.03。
描述了一种用于婴儿配方食品和成人营养物质中牛磺酸分析的亲水相互作用液相色谱-串联质谱（HILIC-MS/MS）方法。一项单实验室验证（SLV）研究表明，该方法适用于满足 2014.013 年 SMPR 的要求。2022 年 12 月，SPIFAN ERP 投票通过该方法作为第一行动 AOAC 官方方法 2022.03。
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