Single-Cell Exogenous Gene Transfection Analysis Chip.

Fast and accurate profiling of exogenous gene expression in host cells is crucial for studying gene function in cellular and molecular biology. This is achieved by co-expression of target genes and reporter genes, but we still have to face the challenge of incomplete co-expression of the reporter and target genes. Here, we present a single-cell transfection analysis chip (scTAC), which is based on the in situ microchip immunoblotting method, for rapid and accurate analysis of exogenous gene expression in thousands of individual host cells. scTAC not only can assign information of exogenous gene activity to specific transfected cells but can also enable the acquisition of continuous protein expression even in incomplete and low co-expression scenarios.