结直肠癌中 POFUT1 作用机制的初步研究。
Preliminary study on the mechanism of POFUT1 in colorectal cancer.
机构信息
Department of Histology & Embryology, Xiangya School of Medicine, Central South University, ChangSha, China.
Yueyang Key Laboratory of Chronic Noncommunicable Diseases, Yueyang Vocational and Technical College, Yueyang, China.
出版信息
Med Oncol. 2023 Jul 11;40(8):235. doi: 10.1007/s12032-023-02102-w.
To analyse the effect of POFUT1 (Protein O-Fucosyltransferase 1) on the proliferation, migration and apoptosis of colorectal cancer (CRC) cells and to explore its potential mechanism. The effects of POFUT1 silencing in vitro on the proliferation, migration, and apoptosis of CRC cells were investigated using the SW480 and RKO cell lines. The effect of POFUT1 expression on cell phenotype was detected by cell proliferation assay (CCK8), colony formation assay, flow cytometry, wound healing assay, transwell assay, cell apoptosis assay, etc. In vitro, silencing of POFUT1 resulted in decreased proliferation, cell cycle arrest, reduced migration and increased apoptosis of CRC cells. In CRC cells, POFUT1 plays a tumour-promoting role by promoting cell proliferation and migration and inhibiting apoptosis.
分析蛋白 O-岩藻糖基转移酶 1（POFUT1）对结直肠癌细胞增殖、迁移和凋亡的影响，并探讨其潜在机制。采用 SW480 和 RKO 细胞系，体外沉默 POFUT1 观察其对 CRC 细胞增殖、迁移和凋亡的影响。通过细胞增殖实验（CCK8）、集落形成实验、流式细胞术、划痕愈合实验、Transwell 实验、细胞凋亡实验等检测 POFUT1 表达对细胞表型的影响。体外实验中，沉默 POFUT1 可降低 CRC 细胞的增殖、细胞周期停滞、迁移减少和凋亡增加。在 CRC 细胞中，POFUT1 通过促进细胞增殖和迁移、抑制细胞凋亡，发挥肿瘤促进作用。
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