Hasan Nabeel Rashid, Dehuri Priyadarshini, Jena Amitabh, Kanungo Srikanta
Department of Pathology, IMS and SUM Hospital, Bhubaneswar, Odisha, India.
Surgical Oncology, IMS and SUM Hospital, Bhubaneswar, Odisha, India.
J Microsc Ultrastruct. 2023 Jan 5;11(2):97-102. doi: 10.4103/jmau.jmau_138_20. eCollection 2023 Apr-Jun.
We undertook this study in view of the rising interest in the interactions between tumor cells and mast cells in the tumor microenvironment and the mounting evidence of the role of mast cells in the prognosis of various cancers.
The aim of the study is to determine the prognostic utility of mast cells and their mediators in oral squamous cell carcinoma (OSCC).
This is a cross-sectional analytical study. A total of 41 cases were studied for determining the correlation of mast cell counts with histopathological prognostic variables and 29 cases for determining the serum histamine levels and to further correlate the values with the histopathologic determinants.
Mast cell assessment was done using Toluidine blue stain on tissue sections while histopathological prognostic factors were evaluated with routine stains. The serum histamine levels were obtained by sandwich enzyme linked immunosorbent assay (ELISA).
Mann-Whitney -test was used to test the difference between parameters and a Spearman correlation coefficient was used. < 0.05 was considered significant for statistical analysis.
We found statistically significant correlations between increased median mast cell counts and higher grade of tumor, presence of lymphovascular invasion, greater depth of invasion, and presence of regional lymph node involvement. The serum histamine values correlated only with the depth of tumor invasion.
Hence, as per our results, we suggest the use of mast cell counts in OSCC cases as a prognostic indicator in conjunction with other clinicopathological factors. At the same time, we acknowledge the fact that extensive studies are required to establish the role of mast cell mediators in the prognostic curve of oral cancer patients.
鉴于肿瘤微环境中肿瘤细胞与肥大细胞之间的相互作用越来越受到关注，且越来越多的证据表明肥大细胞在各种癌症的预后中发挥作用，我们开展了这项研究。
本研究的目的是确定肥大细胞及其介质在口腔鳞状细胞癌（OSCC）中的预后价值。
这是一项横断面分析研究。共研究了41例病例以确定肥大细胞计数与组织病理学预后变量之间的相关性，29例病例用于确定血清组胺水平，并进一步将这些值与组织病理学决定因素相关联。
在组织切片上使用甲苯胺蓝染色进行肥大细胞评估，同时用常规染色评估组织病理学预后因素。血清组胺水平通过夹心酶联免疫吸附测定（ELISA）获得。
采用曼-惠特尼检验来检验参数之间的差异，并使用斯皮尔曼相关系数。P<0.05被认为具有统计学意义。
我们发现，肥大细胞计数中位数增加与肿瘤分级较高、存在淋巴管侵犯、浸润深度较大以及存在区域淋巴结受累之间存在统计学上的显著相关性。血清组胺值仅与肿瘤浸润深度相关。
因此，根据我们的结果，我们建议在OSCC病例中，将肥大细胞计数与其他临床病理因素结合起来作为预后指标。同时，我们承认需要进行广泛的研究来确定肥大细胞介质在口腔癌患者预后曲线中的作用。
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