SBÜ Bağcılar Eğitim ve Araştırma Hastanesi Ortopedi ve Travmatoloji Kliniği, 34200 Bağcılar, İstanbul, Türkiye.
Jt Dis Relat Surg. 2023 Apr 26;34(2):413-424. doi: 10.52312/jdrs.2023.994.
The aim of this study was to examine the effects of cephalexin on the fracture union histomorphometrically, radiologically, biomechanically, immunohistochemically, and histopathologically in a rat femur fracture model and to evaluate the effects of the antibiotics to be used in the prophylaxis of fracture infection on the union of the fracture.
A total of 48 male Wistar rats were divided into four groups as two-week control (C2) and cephalexin (CEP2) and four-week control (C4) and cephalexin (CEP4). After establishment of standard fracture model on right femurs, 60 mg/kg/day of cephalexin was applied to CEP2 and CEP4 by oral gavage. Radiological, biomechanical, histopathological, immunohistochemical, and histomorphometric examinations were performed on amputated femurs.
Callus volume of CEP4 group significantly increased compared to CEP2 group (p=0.005), while no significant difference was found in the bone mineral density and callus/bone volume among the groups (p>0.05). There was no significant difference in flexural strength between the C4 and CEP4 groups (p=0.093). Histological healing scores increased from Week 2 to Week 4 (p=0.002) and inflammation scores decreased in both control and cephalexin groups (p=0.010 and p=0.008); however, no significant difference was found in healing and inflammation scores (p>0.05). The CD34+ immunoreactivity in the CEP2 group was significantly higher than the C2 group (p=0.029). Collagen type III level was significantly lower in the CEP2 and CEP4 groups compared to the corresponding control groups (p=0.008 and p=0.016, respectively).
Cephalexin did not exert any radiological, histopathological, histomorphometric, biomechanical, and immunohistochemical adverse effects on the femoral fracture healing model in rats; however, it showed positive effects on CD34 and Collagen type III levels. Based on these findings, antibiotherapy with cephalexin may be considered as a safe treatment for fracture union.
本研究旨在通过大鼠股骨骨折模型，从组织形态计量学、影像学、生物力学、免疫组织化学和组织病理学方面研究头孢氨苄对骨折愈合的影响，并评估拟用于预防骨折感染的抗生素对骨折愈合的影响。
共 48 只雄性 Wistar 大鼠分为两组，即两周对照组（C2）和头孢氨苄组（CEP2）和四周对照组（C4）和头孢氨苄组（CEP4）。在右侧股骨建立标准骨折模型后，通过口服灌胃给予 CEP2 和 CEP4 组 60mg/kg/天的头孢氨苄。对截肢股骨进行影像学、生物力学、组织病理学、免疫组织化学和组织形态计量学检查。
CEP4 组的骨痂体积明显大于 CEP2 组（p=0.005），而各组的骨矿物质密度和骨痂/骨体积无显著差异（p>0.05）。C4 组和 CEP4 组的弯曲强度无显著差异（p=0.093）。组织学愈合评分从第 2 周增加到第 4 周（p=0.002），两组的炎症评分均降低（p=0.010 和 p=0.008）；然而，愈合和炎症评分无显著差异（p>0.05）。CEP2 组的 CD34+免疫反应性明显高于 C2 组（p=0.029）。与相应的对照组相比，CEP2 和 CEP4 组的 III 型胶原水平明显降低（p=0.008 和 p=0.016）。
头孢氨苄对大鼠股骨骨折愈合模型无放射学、组织病理学、组织形态计量学、生物力学和免疫组织化学不良影响；然而，它对 CD34 和 III 型胶原水平有积极影响。基于这些发现，头孢氨苄的抗生素治疗可能被认为是一种安全的骨折愈合治疗方法。
Zotero 插件