Screening differential circular RNA expression profiles and the potential role of in liver cancer.
作者信息
Zhou Churen, Zhu Duo, Zhou Sibin, Wang Haofan, Huang Mingsheng
机构信息
Department of Interventional Radiology Key Laboratory of Liver Disease Research, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Department of Interventional Radiology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
出版信息
J Cancer Res Ther. 2023 Apr-Jun;19(3):548-555. doi: 10.4103/jcrt.jcrt_1868_20.
AIMS
This study aimed to screen the circular RNAs (circRNAs) that are differentially expressed between liver cancer and paired paracarcinoma tissues and then elucidate their role in cancer progression.
MATERIALS AND METHODS
High-throughput sequencing of cancer and paired paracarcinoma tissues was followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the parental genes of the differentially expressed circRNAs, which were also verified via real-time quantitative polymerase chain reaction analysis of the tissues. In addition, the function of selected circRNAs was determined using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)-2H-tetrazolium (MTS) and transwell assays.
RESULTS
Total 218 and 121 circRNAs were differentially upregulated and downregulated, respectively; these were mainly enriched with GO and KEGG terms related to biological functions. From five representatives of the differentially expressed circRNAs, we selected hsa_circ_0085465 for further analysis, discovering that its overexpression promoted the proliferation, migration, and invasion of 97 L cells.
CONCLUSION
Taken together, our results suggest that hsa_circ_0085465 is relevant to liver cancer progression.
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