糖枫叶片年度生长周期中的酚类物质分布及其与抗氧化活性的关系。

Phenolic mapping and associated antioxidant activities through the annual growth cycle of sugar maple leaves.

机构信息

Department of Bioresource Engineering, McGill University, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada; Department of Food Technology and Rural Industries, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

Department of Bioresource Engineering, McGill University, Sainte-Anne-de-Bellevue, Quebec H9X 3V9, Canada.

出版信息

Food Chem. 2023 Dec 1;428:136882. doi: 10.1016/j.foodchem.2023.136882. Epub 2023 Jul 16.

DOI:10.1016/j.foodchem.2023.136882

PMID:37481905

Abstract

Concentrations of antioxidant components (analyzed by HPLC-UV) and antioxidant attributes (assayed by radical scavenging and non-radical redox potential methods) of sugar maple leaves (SML) from different harvesting times were investigated. Moreover, measurements of colorimetry, SEM, and FTIR spectroscopy-based characterization of leaves composition, throughout the growth cycle, were performed. Results showed that the antioxidant activities of SML are strongly correlated with phenolic contents and significantly (p < 0.05) varied with harvesting time where minimum amount of total phenolics (105.67 ± 13.16 mg GAE/g DM) and total flavonoids (3.27 ± 0.26 mg CTE/g DM) were found to be concentrated in Fall leaves. The absorption bands obtained from FTIR spectra revealed the presence of functional groups that have great significance towards the antioxidant activity of SML. Principal component analysis revealed that biosynthesis of maximum phenolic compounds in SML mostly occurs during the leaf expansion and growth phases. The obtained data provided a better understanding towards the effect of harvesting time on the phenolic mapping of SML in favor of its valorization into functional food ingredients.

摘要

不同收获时间的糖枫（SML）叶片的抗氧化成分浓度（通过 HPLC-UV 分析）和抗氧化特性（通过自由基清除和非自由基氧化还原电势方法测定）进行了研究。此外，还对叶片成分的比色法、SEM 和基于 FTIR 光谱的特性进行了测量，以了解其在整个生长周期内的变化情况。结果表明，SML 的抗氧化活性与酚类含量密切相关，并且与收获时间显著相关（p < 0.05），其中秋季叶片中总酚（105.67 ± 13.16 mg GAE/g DM）和总类黄酮（3.27 ± 0.26 mg CTE/g DM）的含量最低。FTIR 光谱得到的吸收带表明存在具有重要意义的功能基团，这些基团对 SML 的抗氧化活性有很大的影响。主成分分析表明，SML 中最大量酚类化合物的生物合成主要发生在叶片扩展和生长阶段。获得的数据更好地了解了收获时间对 SML 酚类物质分布的影响，有利于将其作为功能性食品成分进行开发利用。