State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China; National Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou, Guangdong, China.
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China.
J Adv Res. 2024 Jun;60:1-12. doi: 10.1016/j.jare.2023.07.009. Epub 2023 Jul 25.
Preventing crop yield loss caused by pests is critical for global agricultural production. Agricultural pest control has largely relied on chemical pesticides. The interaction between insecticide resistance and the adaptation of herbivorous pests to host plants may represent an emerging threat to future food security.
This study aims to unveil genetic evidence for the reduction in the profitability of resistant cultivars derived from insecticide resistance in target pest insects.
An experimental evolution system encompassing resistant rice and its major monophagous pest, the brown planthopper Nilaparvata lugens, was constructed. Whole genome resequencing and selective sweep analysis were utilized to identify the candidate gene loci related to the adaptation. RNA interference and induced expression assay were conducted to validate the function of the candidate loci.
We found that the imidacloprid-resistant population of N. lugens rapidly adapted to resistant rice IR36. Gene loci related to imidacloprid resistance may contribute to this phenomenon. Multiple alleles in the nicotinic acetylcholine receptor (nAChR)-7-like and P450 CYP4C61 were significantly correlated with changes in virulence to IR36 rice and insecticide resistance of N. lugens. One avirulent/susceptible genotype and two virulent/resistant genotypes could be inferred from the corresponding alleles. Importantly, we found that the virulent/resistant genotypes already exist in the wild in China, exhibiting increasing frequencies along with insecticide usage. We validated the relevance of these genotypes and the virulence to three more resistant rice cultivars. Knockdown of the above two genes in N. lugens significantly decreased both the resistance to imidacloprid and the virulence towards resistant rice.
Our findings provide direct genetic evidence to the eco-evolutionary consequence of insecticide resistance, and suggest an urgent need for the implementation of predictably sustainable pest management.
防止害虫导致的作物减产对全球农业生产至关重要。农业害虫防治在很大程度上依赖于化学农药。杀虫剂抗性的出现以及植食性害虫对宿主植物的适应性可能代表着未来粮食安全的新兴威胁。
本研究旨在揭示目标害虫中杀虫剂抗性导致的抗性品种盈利能力降低的遗传证据。
构建了一个包含抗虫水稻及其主要单食性害虫褐飞虱 Nilaparvata lugens 的实验进化系统。利用全基因组重测序和选择清除分析，鉴定与适应相关的候选基因座。通过 RNA 干扰和诱导表达测定，验证候选基因座的功能。
我们发现，褐飞虱对吡虫啉的抗性种群迅速适应了抗虫水稻 IR36。与吡虫啉抗性相关的基因座可能促成了这一现象。烟碱型乙酰胆碱受体（nAChR）-7 样和 P450 CYP4C61 中的多个等位基因与 IR36 水稻的毒力变化和褐飞虱对杀虫剂的抗性显著相关。可以从相应的等位基因推断出一种无毒/敏感基因型和两种有毒/抗性基因型。重要的是，我们发现这些有毒/抗性基因型在中国的野外已经存在，并且随着杀虫剂的使用频率增加而增加。我们验证了这些基因型与三种更多抗虫水稻品种的相关性和毒力。褐飞虱中上述两个基因的敲低显著降低了对吡虫啉的抗性和对抗虫水稻的毒力。
本研究结果为杀虫剂抗性的生态进化后果提供了直接的遗传证据，并表明迫切需要实施可预测的可持续害虫管理。
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