通过下一代测序技术快速诊断致死性菌血症性肺炎和脓胸：一例病例报告
Rapid diagnosis of fatal bacteremic pneumonia and empyema thoracis by next-generation sequencing: a case report.
作者信息
Xing Fanfan, Xia Yao, Lu Qianyun, Lo Simon K F, Lau Susanna K P, Woo Patrick C Y
机构信息
Department of Clinical Microbiology and Infection Control, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
出版信息
Front Med (Lausanne). 2023 Jul 18;10:1226126. doi: 10.3389/fmed.2023.1226126. eCollection 2023.
species do not replicate as rapidly as other pyogenic bacteria and nocardial infections can be highly fatal, particularly in immunocompromised patients. Here, we present the first report of fatal bacteremic pneumonia and empyema thoracis diagnosed by next-generation sequencing (NGS) using the Oxford Nanopore Technologies' MinION device. The bacterium was not identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Due to its low equipment cost, short turn-around-time, and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.
该菌种不像其他化脓性细菌那样快速繁殖，诺卡菌感染可能具有高度致命性，尤其是在免疫功能低下的患者中。在此，我们报告首例使用牛津纳米孔技术公司的MinION设备通过下一代测序（NGS）诊断的致命性菌血症性肺炎和脓胸。基质辅助激光解吸电离飞行时间质谱未鉴定出该细菌。由于设备成本低、周转时间短且体积便携，牛津纳米孔技术公司的MinION设备是常规临床微生物实验室进行NGS的有用平台。
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