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斑马鱼脊髓中血管的发育和修复。

Development and repair of blood vessels in the zebrafish spinal cord.

机构信息

Instituto de Medicina Molecular-João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, Lisboa 1649-028 Portugal.

Centro Cardiovascular da Universidade de Lisboa (CCUL@RISE), Faculdade de Medicina da Universidade de Lisboa, Lisboa 1649-028 Portugal.

出版信息

Open Biol. 2023 Aug;13(8):230103. doi: 10.1098/rsob.230103. Epub 2023 Aug 9.

DOI:10.1098/rsob.230103
PMID:37553073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10409570/
Abstract

The vascular system is inefficiently repaired after spinal cord injury (SCI) in mammals, resulting in secondary tissue damage and immune deregulation that contribute to the limited functional recovery. Unlike mammals, zebrafish can repair the spinal cord (SC) and restore motility, but the vascular response to injury has not been investigated. Here, we describe the zebrafish SC blood vasculature, starting in development with the initial vessel ingression in a body size-dependent manner, the acquisition of perivascular support and the establishment of ventral to dorsal blood circulation. The vascular organization grows in complexity and displays multiple barrier specializations in adulthood. After injury, vessels rapidly regrow into the lesion, preceding the glial bridge and axons. Vascular repair involves an early burst of angiogenesis that creates dysmorphic and leaky vessels. Dysfunctional vessels are later removed, as pericytes are recruited and the blood-SC barrier is re-established. This study demonstrates that zebrafish can successfully re-vascularize the spinal tissue, reinforcing the value of this organism as a regenerative model for SCI.

摘要

血管系统在哺乳动物的脊髓损伤 (SCI) 后修复效率低下，导致继发性组织损伤和免疫失调，从而限制了功能恢复。与哺乳动物不同，斑马鱼可以修复脊髓 (SC) 并恢复运动能力，但血管对损伤的反应尚未被研究。在这里，我们描述了斑马鱼 SC 的血管系统，从发育开始，血管以依赖于体型的方式最初进入，获得血管周围支持并建立从腹侧向背侧的血液循环。血管组织的复杂性不断增加，并在成年期显示出多种屏障特化。损伤后，血管迅速向损伤部位再生，先于神经胶质桥和轴突。血管修复涉及早期血管生成的爆发，形成畸形和渗漏的血管。功能失调的血管随后被清除，因为周细胞被招募，血脊髓屏障被重新建立。这项研究表明，斑马鱼可以成功地使脊髓组织重新血管化，这加强了该生物体作为 SCI 再生模型的价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/69e7d21b3ab1/rsob230103f09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/56bf96801cf1/rsob230103f01.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/f992b4453858/rsob230103f08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/69e7d21b3ab1/rsob230103f09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/56bf96801cf1/rsob230103f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/4d28825b3aa7/rsob230103f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/8051edd2b770/rsob230103f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/2ff171694124/rsob230103f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/fa5d2de244b1/rsob230103f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/f96923b2eff5/rsob230103f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/00f4021ef0da/rsob230103f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/f992b4453858/rsob230103f08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cfb/10409570/69e7d21b3ab1/rsob230103f09.jpg

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