Singer A G, Macrides F, Clancy A N, Agosta W C
J Biol Chem. 1986 Oct 5;261(28):13323-6.
Hormonally regulated proteinaceous material secreted in hamster vaginal discharge is detected via the vomeronasal organ and elicits copulatory behavior in males. The major soluble protein in estrous vaginal discharge has been isolated, characterized by molecular weight and amino acid content, and shown to have substantial aphrodisiac activity. The aphrodisiac activity of the purified protein is abolished by heating or proteolysis, and the native protein retains the activity after procedures for removing possible ligands such as volatile odorants, steroids, and peptides. This evidence that the protein is a reproductive pheromone indicates that the mammalian vomeronasal organ can mediate sensory detection of behaviorally relevant macromolecules.
在仓鼠阴道分泌物中分泌的受激素调节的蛋白质物质通过犁鼻器被检测到，并引发雄性的交配行为。发情期阴道分泌物中的主要可溶性蛋白质已被分离出来，通过分子量和氨基酸含量进行了表征，并显示出具有显著的催情活性。纯化后的蛋白质经加热或蛋白水解后催情活性消失，而天然蛋白质在去除可能的配体（如挥发性气味物质、类固醇和肽）的处理后仍保留活性。这种蛋白质是一种生殖信息素的证据表明，哺乳动物的犁鼻器可以介导对行为相关大分子的感官检测。
