Green inspired synthesis of zinc oxide nanoparticles using (milk thistle) extract and evaluation of their potential pesticidal and phytopathogens activities.

BACKGROUND

The green approaches for the synthesis of nanoparticles are gaining significant importance because of their high productivity, purity, low cost, biocompatibility, and environmental friendliness.

METHODS

The aim of the current study is the green synthesis of zinc oxide nanoparticles (ZnO-NPs) using seed extracts of , which acts as a reducing and stabilizing agent. central composite design (CCD) of response surface methodology (RSM) optimized synthesis parameters (temperature, pH, reaction time, plant extract, and salt concentration) for controlled size, stability, and maximum yields of ZnO-NPs. Green synthesized ZnO-NPs was characterized using UV-visible spectroscopy and Zetasizer analyses.

RESULTS

The Zetasizer confirmed that green synthesized ZnO-NPs were 51.80 nm in size and monodispersed in nature. The UV-visible results revealed a large band gap energy in the visible region at 360.5 nm wavelength. The bioactivities of green synthesized ZnO-NPs, including antifungal, antibacterial, and pesticidal, were also evaluated. Data analysis confirmed that these activities were concentration dependent. Bio-synthesized ZnO-NPs showed higher mortality towards of about 78 ± 0.57% after 72 h observation as compared to , which only displayed 74 ± 0.57% at the same concentration and time intervals. Plant-mediated ZnO-NPs also showed high potential against pathogenic gram-positive bacteria (), gram-negative bacteria (), and two fungal strains such as , and A with inhibition zones of 18 ± 0.4, 25 ± 0.4, 21 ± 0.57, and 19 ± 0.4 mm, respectively.

CONCLUSION

The results of this study showed that -based ZnO-NPs are cost-effective and efficient against crop pests.