用于确诊麻疹的特异性免疫球蛋白M酶联免疫吸附测定
Specific immunoglobulin M enzyme-linked immunosorbent assay for confirming the diagnosis of measles.
作者信息
Lievens A W, Brunell P A
出版信息
J Clin Microbiol. 1986 Sep;24(3):391-4. doi: 10.1128/jcm.24.3.391-394.1986.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was evaluated for the detection of measles virus-specific immunoglobulin M (IgM) (MIgM). The ELISA was standardized by deriving a seronegative range of values from sera which should not contain MIgM (24 cord sera, 59 sera from immune health care workers, and 47 sera from infants before the administration of measles vaccine). These values were separable from those obtained from individuals convalescing from measles. Twenty sera containing rheumatoid factor were MIgM seronegative. Of 30 acute-phase sera from suspected measles cases, 26 contained MIgM; those that were seronegative were obtained on day 0, 0, 2, or 9. All 25 convalescent-phase samples contained MIgM. Of the 25 paired samples, 22 were IgG positive at the first sampling; 3 of the 22 did not show a rise in IgG titer. The MIgM ELISA can be used for confirming suspected measles cases, often requiring only a single serum specimen.
摘要
对一种酶联免疫吸附测定法（ELISA）进行了评估，用于检测麻疹病毒特异性免疫球蛋白M（IgM）（MIgM）。通过从不应含有MIgM的血清（24份脐带血清、59份来自免疫医护人员的血清以及47份在接种麻疹疫苗前的婴儿血清）中得出血清阴性值范围，对ELISA进行了标准化。这些值与从麻疹恢复期个体获得的值可区分开来。20份含有类风湿因子的血清MIgM呈血清阴性。在30份来自疑似麻疹病例的急性期血清中，26份含有MIgM；血清阴性的样本是在第0天、第0天、第2天或第9天采集的。所有25份恢复期样本均含有MIgM。在25对配对样本中，22份在首次采样时IgG呈阳性；这22份中有3份未显示IgG滴度升高。MIgM ELISA可用于确诊疑似麻疹病例，通常仅需一份血清样本。
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