Xu Nan, Du Xin, Zhang Xiu-Xing, Yang Hai-Ling
The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration; Institute of Tree Development and Genome Editing, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.
Mitochondrial DNA B Resour. 2023 Aug 21;8(8):877-881. doi: 10.1080/23802359.2023.2246675. eCollection 2023.
Wallich ex Andersson 1851 is a species of genus which mainly grows on mountains above 3000 m at sea level in Qinghai-Tibetan Plateau (including the Himalayas and Hengduan Mountains). To determine its phylogenetic position within , we reconstructed complete chloroplast (cp) genome sequence by de novo assembly using whole-genome sequencing data. The completed chloroplast genome was 155,304 bp, with a total GC content of 36.7%. It had a very typical tetrad structure, including a large single-copy (LSC) region of 84,539 bp, a small single-copy (SSC) region of 16,161 bp, and two inverted repeats (IR) regions of 27,302 bp. A total of 132 functional genes were distributed in the chloroplast genome, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis showed that was clustered with Wimmer 1849 and Franchet 1887. The complete chloroplast genome of provides potential genetic resources for further phylogenetic studies.
Wallich ex Andersson 1851是一种主要生长在青藏高原海拔3000米以上山区（包括喜马拉雅山脉和横断山脉）的属种。为确定其在该属内的系统发育位置，我们利用全基因组测序数据通过从头组装重建了完整的叶绿体（cp）基因组序列。完成的叶绿体基因组为155,304 bp，总GC含量为36.7%。它具有非常典型的四分体结构，包括一个84,539 bp的大单拷贝（LSC）区域、一个16,161 bp的小单拷贝（SSC）区域和两个27,302 bp的反向重复（IR）区域。叶绿体基因组中共分布有132个功能基因，包括87个蛋白质编码基因、37个tRNA基因和8个rRNA基因。系统发育分析表明，该物种与Wimmer 1849和Franchet 1887聚类在一起。该物种完整的叶绿体基因组为进一步的系统发育研究提供了潜在的遗传资源。
