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动物毛癣病灭活疫苗的研制与应用。

The development and use of an inactivated vaccine against animals trichophytosis.

机构信息

Department of Biological Safety, Kazakh National Agrarian Research University, Almaty, Republic of Kazakhstan.

Department of Obstetrics, Surgery and Biotechnology of Reproduction of Animals, Kazakh National Agrarian Research University, Almaty, Republic of Kazakhstan.

出版信息

Open Vet J. 2023 Jul;13(7):864-872. doi: 10.5455/OVJ.2023.v13.i7.8. Epub 2023 Jul 31.

Abstract

BACKGROUND

The annual increase in the number of camels entails a parallel increase in the incidence of trichophytosis, which poses a great threat to the health and life of both this species of animals and other organisms that contact and surround them.

AIM

The aim of the study was to develop and establish the quality of vaccines inactivated by ultrasonic exposure for the prevention and treatment of trichophytosis in camels, and to compare them with chemically deactivated vaccines.

METHODS

The peculiarity of the technology of production of these vaccines was the use of an innovative method of inactivation of fungal strains by ultrasonic waves, which allowed to achieve high positive results in theory, and was subsequently confirmed in practice by immunizing sick and healthy animals. The first tests of the obtained vaccines were conducted in laboratory conditions on experimental rabbits.

RESULTS

The results of prophylactic and therapeutic vaccinations were one hundred percent positive, which made it possible to conduct further tests directly on camels of industrial farms, the expected result of which was also positively confirmed at the end of the research.

CONCLUSION

As a result of this experiment, the effectiveness, stability, and safety of the manufactured vaccines were established, which made it possible to approve the regulatory and technical documentation and patent them as an innovative and effective development for the prevention and treatment of camel trichophytosis, which will reduce the growth of infection and further overcome the mass spread of the disease both among camels and among the surrounding organisms to which it is transmitted.

摘要

背景

骆驼数量的逐年增加导致毛癣菌病的发病率也相应增加,这对该物种动物的健康和生命以及与之接触和周围的其他生物构成了极大的威胁。

目的

本研究旨在开发和建立超声暴露灭活疫苗的质量,用于预防和治疗骆驼毛癣菌病,并将其与化学灭活疫苗进行比较。

方法

这些疫苗生产技术的特点是采用创新的超声波方法灭活真菌株,这在理论上可以达到很高的阳性结果,随后通过对患病和健康动物进行免疫接种在实践中得到了证实。获得的疫苗的初步测试是在实验兔身上进行的实验室条件下进行的。

结果

预防性和治疗性接种的结果均为百分之百阳性,这使得可以直接在工业农场的骆驼身上进行进一步的测试,研究结束时也得到了积极的确认。

结论

通过这项实验,确定了所制造疫苗的有效性、稳定性和安全性,从而获得了监管和技术文件的批准,并将其作为一种预防和治疗骆驼毛癣菌病的创新有效方法进行了专利注册,这将减少感染的传播,并进一步克服该病在骆驼和传播给它的周围生物中的大规模传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e2/10443827/6495d82ced74/OpenVetJ-13-864-g001.jpg

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