Stomatological Hospital of Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing 401147, P. R. China.
ACS Biomater Sci Eng. 2023 Sep 11;9(9):5332-5346. doi: 10.1021/acsbiomaterials.3c00454. Epub 2023 Aug 29.
Periodontitis is an inflammatory disease characterized by tooth loss and alveolar bone resorption. Bacteria are the original cause of periodontitis, and excess reactive oxygen species (ROS) encourage and intensify inflammation. In this study, a mussel-inspired and MnO NPs-reinforced adhesive hydrogel capable of alleviating periodontitis with improved antibacterial and antioxidant abilities was developed. The hydrogel was created by combining polyvinyl alcohol (PVA), 3,4-dihydroxy-d-phenylalanine (DOPA), and MnO nanoparticles (NPs) (named PDMO hydrogel). The hydrogel was demonstrated to be able to scavenge various free radicals (including total ROS─O and OH) and relieve the hypoxia in an inflammatory microenvironment by scavenging excess ROS and generating O due to its superoxide dismutase (SOD)/catalase (CAT)-like activity. Besides, under 808 nm near-infrared (NIR) light, the photothermal performance of the PDMO hydrogel displayed favorable antibacterial and antibiofilm effects toward , , and (up to nearly 100% antibacterial rate). Furthermore, the PDMO hydrogel exhibited favorable therapeutic efficacy in alleviating gingivitis in Sprague-Dawley rats, even comparable to or better than the commercial PERIO. In addition, in the periodontitis models, the PDMO2 group showed the height of the residual alveolar bone and the smallest shadow area of low density among other groups, indicating the positive role of the PDMO2 hydrogel in bone regeneration. Finally, the biosafety of the PDMO hydrogel was comprehensively investigated, and the hydrogel was demonstrated to have good biocompatibility. Therefore, the developed PDMO hydrogel provided an effective solution to resolve biofilm recolonization and oxidative stress in periodontitis and could be a superior candidate for local drug delivery system in the clinical management of periodontitis with great potential for future clinical translation.
牙周炎是一种以牙齿丧失和牙槽骨吸收为特征的炎症性疾病。细菌是牙周炎的原始病因，过量的活性氧（ROS）促进并加剧炎症。在这项研究中，开发了一种受贻贝启发且 MnO NPs 增强的黏附水凝胶，具有改善的抗菌和抗氧化能力，可缓解牙周炎。该水凝胶由聚乙烯醇（PVA）、3,4-二羟基-D-苯丙氨酸（DOPA）和 MnO 纳米颗粒（NPs）制成（命名为 PDMO 水凝胶）。该水凝胶通过清除过量的 ROS 并产生 O，具有清除各种自由基（包括总 ROS─O 和 OH）和减轻炎症微环境缺氧的能力，因为其具有超氧化物歧化酶（SOD）/过氧化氢酶（CAT）样活性。此外，在 808nm 近红外（NIR）光下，PDMO 水凝胶的光热性能对 、 、 和 （抗菌率高达近 100%）表现出良好的抗菌和抗生物膜作用。此外，PDMO 水凝胶在缓解 Sprague-Dawley 大鼠牙龈炎方面表现出良好的治疗效果，甚至可与商业 PERIO 相媲美或更好。此外，在牙周炎模型中，PDMO2 组显示出残留牙槽骨的高度和低密度阴影区域最小，表明 PDMO2 水凝胶在骨再生中发挥了积极作用。最后，全面研究了 PDMO 水凝胶的生物安全性，结果表明水凝胶具有良好的生物相容性。因此，开发的 PDMO 水凝胶为解决牙周炎中的生物膜再定植和氧化应激提供了有效解决方案，并且可能成为牙周炎临床管理中局部药物输送系统的优秀候选者，具有巨大的临床转化潜力。
