Faculty of Pharmacy, Department of Pharmacognosy, Gazi University, 06330, Ankara, Turkey.
Department of Biology, Faculty of Science, Gazi University, Etiler, 06330, Ankara, Turkey.
J Ethnopharmacol. 2024 Jan 30;319(Pt 1):117122. doi: 10.1016/j.jep.2023.117122. Epub 2023 Sep 1.
Cistus laurifolius is widely used in folk medicine in Anatolia for the treatment of many ailments. The leaves of the plant are used in the form of tea in the treatment of hemorrhoids in the Western Black Sea Region and Central Anatolia.
It was aimed at evaluating the anti-hemorrhoidal effects of C. laurifolus leaves in croton oil-induced hemorrhoid model in rats.
The methanolic and aqueous extracts of C. laurifolius were tested for in vivo anti-hemorrhoidal efficacy using an experimental hemorrhoid model, followed by histological and biochemical analysis. Hemorrhoid was created by using croton oil on the anal region of the rats. TNF-α and VEGF mRNA expression levels were assessed using real-time PCR detections. The extract was also tested for anti-inflammatory properties, which are based on the suppression of an increase in capillary permeability caused by acetic acid. LC-QTOF-MS and RP-HPLC were used for the phytochemical analysis.
In comparison to the control, histological and biochemical assessment showed that the methanolic extract of C. laurifolius is particularly effective against hemorrhoids. The same extract group's TNF-α mRNA expression was found to be the lowest. Additionally, the methanolic extract showed a strong inhibitory effect on the increase in capillary permeability resulted on by acetic acid. Three phenolic compounds were discovered in the extracts by phytochemical analyses, while more than eighteen compounds were found by LC-QTOF-MS analysis. Five of these compounds are phenolic acid derivatives, and flavonoids constitute the majority of the group.
This is the first evidence from the research that C. laurifolius possesses strong anti-inflammatory and anti-hemorrhoidal properties.
在安纳托利亚，Cistus laurifolius 被广泛应用于民间医学，用于治疗多种疾病。该植物的叶子以茶的形式用于治疗西黑海水域和中安纳托利亚的痔疮。
旨在评估 C. laurifolius 叶在大鼠角叉菜胶诱导的痔疮模型中的抗痔疮作用。
使用实验性痔疮模型对 C. laurifolius 的甲醇和水提取物进行体内抗痔疮功效测试，随后进行组织学和生化分析。通过在大鼠肛门区域使用巴豆油来创建痔疮。使用实时 PCR 检测评估 TNF-α 和 VEGF mRNA 表达水平。还测试了提取物的抗炎特性，这是基于抑制由乙酸引起的毛细血管通透性增加。使用 LC-QTOF-MS 和 RP-HPLC 进行植物化学分析。
与对照组相比，组织学和生化评估表明 C. laurifolius 的甲醇提取物对痔疮特别有效。同一提取物组的 TNF-α mRNA 表达最低。此外，该甲醇提取物对乙酸引起的毛细血管通透性增加具有强烈的抑制作用。通过植物化学分析在提取物中发现了三种酚类化合物，而通过 LC-QTOF-MS 分析发现了超过十八种化合物。这些化合物中有五种是酚酸衍生物，而黄酮类化合物构成了该组的大部分。
这是首次有研究证明 C. laurifolius 具有强大的抗炎和抗痔疮特性。
