人免疫和初始 scFv 噬菌体展示文库的构建。

Construction of Human Immune and Naive scFv Phage Display Libraries.

机构信息

Institut für Biochemie, Biotechnologie und Bioinformatik, Departments Biotechnology and Medical Biotechnology, Technische Universität Braunschweig, Braunschweig, Germany.

Abcalis GmbH, Braunschweig, Germany.

出版信息

Methods Mol Biol. 2023;2702:15-37. doi: 10.1007/978-1-0716-3381-6_2.

DOI:10.1007/978-1-0716-3381-6_2

PMID:37679613

Abstract

Antibody phage display is a widely used in vitro selection technology for the generation of human recombinant antibodies and has yielded thousands of useful antibodies for research, diagnostics, and therapy. In order to successfully generate antibodies using phage display, the basis is the construction of high-quality antibody gene libraries. Here, we describe detailed methods for the construction of such high-quality immune and naive scFv gene libraries of human origin. These protocols were used to develop human naive (e.g., HAL9/10) and immune libraries, which resulted in thousands of specific antibodies for all kinds of applications.

摘要

噬菌体展示技术是一种广泛应用于体外筛选的技术，可用于生成人源重组抗体，并已产生了数千种用于研究、诊断和治疗的有用抗体。为了成功地使用噬菌体展示生成抗体，基础是构建高质量的抗体基因文库。在这里，我们描述了构建高质量的人源免疫和原始 scFv 基因文库的详细方法。这些方案用于开发人类原始（例如 HAL9/10）和免疫文库，从而产生了数千种用于各种应用的特异性抗体。