小鼠肝脏神经节苷脂

Mouse liver gangliosides.

作者信息

Suzuki M, Nakamura K, Hashimoto Y, Suzuki A, Yamakawa T

出版信息

Carbohydr Res. 1986 Aug 15;151:213-23. doi: 10.1016/s0008-6215(00)90342-2.

Abstract

The major gangliosides from mouse liver were purified and characterized by t.l.c., g.l.c., sialidase treatment, and a methylation study. GM3(NeuAc), GM3(NeuGc), GM2(NeuGc), GM1(NeuGc), and GDla(NeuGc, NeuGc) were identified. The structural identification of three of the gangliosides, GM2(NeuGc), GM1(NeuGc), and GDla(NeuGc, NeuGc), was supported by the results of 1H-n.m.r. analysis, and the structures of GM3(NeuGc), GM2(NeuGc), and GM1(NeuGc) were further confirmed by negative-ion fast-atom bombardment mass spectrometry. Ganglioside mapping showed that there was polymorphic variation of gangliosides in the liver of inbred strains of mice and that the major gangliosides were GM3(NeuGc) in WHT/Ht, GM2(NeuGc) in BALB/c and C3H/He, and GM2(NeuGc), GM1(NeuGc), and GDla(NeuGc, NeuGc) in ICR mice. Gangliosides containing N-acetylneuraminic acid, except for GM3(NeuAc), were not detected as major gangliosides in the strains of mice we analyzed.

摘要

从小鼠肝脏中纯化出主要神经节苷脂，并通过薄层层析、气相色谱、唾液酸酶处理和甲基化研究对其进行表征。鉴定出了GM3(NeuAc)、GM3(NeuGc)、GM2(NeuGc)、GM1(NeuGc)和GDla(NeuGc, NeuGc)。1H-核磁共振分析结果支持了三种神经节苷脂GM2(NeuGc)、GM1(NeuGc)和GDla(NeuGc, NeuGc)的结构鉴定，负离子快原子轰击质谱进一步证实了GM3(NeuGc)、GM2(NeuGc)和GM1(NeuGc)的结构。神经节苷脂图谱显示，近交系小鼠肝脏中的神经节苷脂存在多态性变化，在WHT/Ht小鼠中主要神经节苷脂是GM3(NeuGc)，在BALB/c和C3H/He小鼠中是GM2(NeuGc)，在ICR小鼠中是GM2(NeuGc)、GM1(NeuGc)和GDla(NeuGc, NeuGc)。在我们分析的小鼠品系中，除GM3(NeuAc)外，含有N-乙酰神经氨酸的神经节苷脂未被检测为主要神经节苷脂。