种子提取物对链脲佐菌素诱导的糖尿病大鼠GLUT-4 mRNA和GLUT-4蛋白的降血糖作用

Hypoglycemic Effect of Seed Extract on GLUT-4 mRNA and GLUT-4 Protein in Streptozotocin-Induced Diabetic Rats.

作者信息

Pipil Neha, Gupta Prem Prakash, Soni Sachin, Chopra Deepti, Lhamo Yangshen, Singh Niraj, Shree Bhagya

机构信息

Department of Pharmacology, All India Institute of Medical Sciences Bilaspur, Himachal Pradesh, India.

Department of Pharmacology, All India Institute of Medical Sciences Patna, Bihar, India.

出版信息

J Pharm Bioallied Sci. 2023 Jul;15(Suppl 2):S1059-S1061. doi: 10.4103/jpbs.jpbs_226_23. Epub 2023 Jul 11.

DOI:10.4103/jpbs.jpbs_226_23

PMID:37693992

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10485492/

Abstract

BACKGROUND

To investigate the effect of N. nucifera hydroalcoholic seed extract on fasting blood glucose (FBG) levels, glucose transporter (GLUT)-4 mRNA, and GLUT-4 protein in the adipose tissue of streptozotocin (STZ)-induced diabetic rats.

MATERIALS AND METHODS

Male Sprague Dawley (SD) rats were first fed with a high-fat diet (HFD) for three weeks, and then, diabetes was induced by intraperitoneal injection of STZ at a dose of 35 mg/kg bw. Rats were divided into four groups: group 1: normal rats (NC), group 2: STZ-induced diabetic rats (DC), group 3: diabetic rats with N. nucifera hydroalcoholic seed extract at a dose of 400 mg/kg bw (NN), and group 4: diabetic rats with metformin at a dose of 100 mg/kg bw (MET) for 28 days.

RESULTS

FBG level was significantly lower in the NN group than in the DC group ( < 0.05). Also, the NN group increased GLUT-4 mRNA expression and GLUT-4 protein in the adipose tissue when compared to the diabetic group.

CONCLUSION

We conclude that the observed hypoglycemic effect of N. nucifera seed extraction in STZ-induced diabetic rats could be due to insulinomimetic activity.

摘要

背景

研究莲种子水醇提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠空腹血糖（FBG）水平、脂肪组织中葡萄糖转运蛋白（GLUT）-4 mRNA和GLUT-4蛋白的影响。

材料与方法

雄性Sprague Dawley（SD）大鼠先喂高脂饮食（HFD）3周，然后腹腔注射35 mg/kg体重的STZ诱导糖尿病。大鼠分为四组：第1组：正常大鼠（NC）；第2组：STZ诱导的糖尿病大鼠（DC）；第3组：给予400 mg/kg体重莲种子水醇提取物的糖尿病大鼠（NN）；第4组：给予100 mg/kg体重二甲双胍的糖尿病大鼠（MET），持续28天。