Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China.
Front Immunol. 2023 Aug 30;14:1208349. doi: 10.3389/fimmu.2023.1208349. eCollection 2023.
() is safe and can be used as vehicle. In this study, the immunoregulatory effect of on dendritic cell (DC) activation and mechanism were investigated. The immune responses and antigen cross-presentation mechanism of DC-based vaccine prepared with OVA recombinant were explored.
Confocal microscopy and flow cytometry were used to analyze the mechanism of promoting DC maturation, phagosome membrane rupture and antigen presentation. The antitumor effect of DC vaccine prepared with was assessed in the B16-OVA tumor mouse model.
significantly promoted DC maturation, which was partially dependent on TLR2 and downstream MAPK and NF-κB signaling pathways. was internalized into DCs by endocytosis and did not co-localized with lysosome. OVA recombinant enhanced antigen cross-presentation of DCs through the phagosome-to-cytosol pathway in a reactive oxygen species (ROS)- and proteasome-dependent manner. In mouse experiments, increased the migration of DCs to draining lymph node and DC vaccine prepared with OVA recombinant induced strong antigen-specific Th1 and cytotoxic T lymphocyte responses, which significantly inhibited B16-OVA tumor growth.
This study demonstrated that recombinant as an antigen delivery system prepared DC vaccine can enhance the antigen cross-presentation and antitumor efficacy.
（）安全且可作为载体。本研究旨在探讨重组（）对树突状细胞（DC）激活的免疫调节作用及其机制。探索用 OVA 重组（）制备的 DC 疫苗的免疫应答和抗原交叉呈递机制。
采用共聚焦显微镜和流式细胞术分析促进 DC 成熟、吞噬体膜破裂和抗原呈递的机制。用（）制备的 DC 疫苗在 B16-OVA 肿瘤小鼠模型中评估其抗肿瘤作用。
（）显著促进 DC 成熟，部分依赖 TLR2 及下游 MAPK 和 NF-κB 信号通路。（）通过内吞作用被内化到 DC 中，与溶酶体不共定位。OVA 重组（）通过吞噬体到细胞质途径，以活性氧（ROS）和蛋白酶体依赖的方式增强 DC 的抗原交叉呈递。在小鼠实验中，（）增加了 DC 向引流淋巴结的迁移，用 OVA 重组（）制备的 DC 疫苗诱导强烈的抗原特异性 Th1 和细胞毒性 T 淋巴细胞应答，显著抑制了 B16-OVA 肿瘤的生长。
本研究表明，作为一种抗原递送系统的重组（）制备的 DC 疫苗可以增强抗原交叉呈递和抗肿瘤功效。
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