诱导 SOX9 猪皮肤来源干细胞向原始生殖细胞样细胞分化。

Induced differentiation of primordial germ cell like cells from SOX9 porcine skin derived stem cells.

机构信息

College of Life Sciences, Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, 266109, China.

Animal Husbandry General Station of Shandong Province, Jinan, 250010, China.

出版信息

Theriogenology. 2023 Dec;212:129-139. doi: 10.1016/j.theriogenology.2023.08.027. Epub 2023 Sep 9.

DOI:10.1016/j.theriogenology.2023.08.027

PMID:37717516

Abstract

Understanding the mechanisms behind porcine primordial germ cell like cells (pPGCLCs) development, differentiation, and gametogenesis is crucial in the treatment of infertility. In this study, SOX9 skin derived stem cells (SOX9 SDSCs) were isolated from fetal porcine skin and a high-purity SOX9 SDSCs population was obtained. The SOX9 SDSCs were induced to transdifferentiate into PGCLCs during 8 days of cultured. The results of RNA-seq, western blot and immunofluorescence staining verified SDSCs have the potential to transdifferentiate into PGCLCs from aspects of transcription factor activation, germ layer differentiation, energy metabolism, and epigenetic changes. Both adherent and suspended cells were collected. The adherent cells were found to be very similar to early porcine primordial germ cells (pPGCs). The suspended cells resembled late stage pPGCs and had a potential to enter meiotic process. This SDSCs culture-induced in vitro model is expected to provide suitable donor cells for stem cell transplantation in the future.

摘要

了解猪原始生殖细胞样细胞（pPGCLCs）的发育、分化和配子发生的机制对于治疗不孕不育至关重要。本研究从胎猪皮肤中分离出 SOX9 皮肤来源干细胞（SOX9 SDSCs），并获得了高纯度的 SOX9 SDSCs 群体。在 8 天的培养过程中，SOX9 SDSCs 被诱导分化为 PGCLCs。RNA-seq、western blot 和免疫荧光染色的结果从转录因子激活、胚层分化、能量代谢和表观遗传变化等方面证实了 SDSCs 具有向 PGCLCs 转分化的潜能。同时收集贴壁细胞和悬浮细胞。贴壁细胞与早期猪原始生殖细胞（pPGCs）非常相似。悬浮细胞类似于晚期 pPGCs，并且有进入减数分裂过程的潜力。这种 SDSCs 培养诱导的体外模型有望为未来的干细胞移植提供合适的供体细胞。