Ivanova Nadezhda, Ermenlieva Neli, Simeonova Lora, Kolev Iliyan, Slavov Iliya, Karashanova Daniela, Andonova Velichka
Department of Pharmaceutical Technologies, Faculty of Pharmacy, Medical University of Varna, 9000 Varna, Bulgaria.
Department of Microbiology and Virology, Faculty of Medicine, Medical University of Varna, 9000 Varna, Bulgaria.
Pharmaceutics. 2023 Sep 9;15(9):2298. doi: 10.3390/pharmaceutics15092298.
This study explored the potential synergism within chlorhexidine-silver nanoparticle conjugates against Influenza type A, , , and . Silver nanoparticles (SN) were obtained by the reduction of silver ions with green tea total phenolic extract and conjugated with chlorhexidine (Cx). The particles were characterized by UV-Vis and FTIR spectroscopies, dynamic light scattering, X-ray diffraction, and transmission electron microscopy. A stable negatively charged nano-silver colloid (ζ = -50.01) was obtained with an average hydrodynamic diameter of 92.34 nm. In the presence of chlorhexidine, the spectral data and the shift of the zeta potential to positive values (ζ = +44.59) revealed the successful sorption of the drug onto the silver surface. The conjugates (SN-Cx) demonstrated potentiation in their effects against and and synergism against with minimal inhibitory concentrations of SN at 5.5 µg/mL + Cx 8.8 µg/mL. The SN showed excellent virucidal properties, increasing with time, and demonstrated low toxicity. However, the coupling of the cationic chlorhexidine with nano-silver did not reduce its intrinsic cytotoxicity on various cell lines (MDCK, BJ, and A549). The newly synthesized antimicrobial agent exhibited an extended and promising therapeutic spectrum and needs to be further evaluated regarding the designated route of administration in three-dimensional cell models (e.g., nasal, bronchial, dermal, ocular, etc.).
本研究探讨了洗必泰 - 银纳米颗粒共轭物对甲型流感病毒的潜在协同作用。通过用绿茶总酚提取物还原银离子获得银纳米颗粒（SN），并使其与洗必泰（Cx）共轭。通过紫外 - 可见光谱和傅里叶变换红外光谱、动态光散射、X射线衍射和透射电子显微镜对颗粒进行表征。获得了一种稳定的带负电荷的纳米银胶体（ζ = -50.01），平均流体动力学直径为92.34 nm。在洗必泰存在的情况下，光谱数据以及zeta电位向正值的转变（ζ = +44.59）表明药物成功吸附到银表面。共轭物（SN - Cx）对[此处原文缺失相关病毒名称]的作用表现出增强，对[此处原文缺失相关病毒名称]表现出协同作用，SN的最小抑菌浓度为5.5 µg/mL + Cx 8.8 µg/mL。SN表现出优异的杀病毒特性，且随时间增加，并且毒性较低。然而，阳离子洗必泰与纳米银的偶联并未降低其对各种细胞系（MDCK、BJ和A549）的内在细胞毒性。新合成的抗菌剂展现出广泛且有前景的治疗谱，需要在三维细胞模型（例如鼻腔、支气管、皮肤、眼部等）中针对指定给药途径进行进一步评估。
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