Department of Anesthesiology, The First College of Clinical Medical Science, China Three Gorges University, Yichang, Hubei, China.
Department of Anesthesiology, Yichang Central People's Hospital, Yichang, Hubei, China.
Immun Inflamm Dis. 2023 Sep;11(9):e1023. doi: 10.1002/iid3.1023.
The heterocyclic compound 4-hydroxy-(2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPOL) has a protective effect on neurological function in brain tissues damaged by ischemia and hypoxia. This study explored the effects of TEMPOL pretreatment on postoperative cognitive function in aged rats under sevoflurane anesthesia, focusing on inflammatory response and oxidative stress.
Sixty male rats were divided into normal control (C), sevoflurane anesthesia (S), TEMPOL pretreatment (T), and sevoflurane anesthesia + TEMPOL pretreatment (ST) groups (15 per group). Groups T and ST rats received continuous intraperitoneal TEMPOL (100 mg/kg) for 3 days, while groups C and S rats were injected with 0.9% saline. After pretreatment, groups S and ST received 3% sevoflurane anesthesia.
Rats in group S exhibited a longer swimming distance, longer escape latency, lower frequency of platform crossing, and shorter dwell time in the targeted quadrant than those in groups C and T. Rats in group ST exhibited a shorter swimming distance, shorter escape latency, higher frequency of platform crossing, and longer dwell time in the targeted quadrant than those in group S. The expressions of interleukin-6, tumor necrosis factor-α, inducible nitric oxide synthase, and Ym1/2 messenger ribonucleic acid were higher in groups S and ST rats than in groups C and T rats and lower in group ST rats than in group S rat (p < .05). Superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px) were lower, while malondialdehyde (MDA) was higher in groups S and ST rats than in groups C and T rats (p < .05). Group ST showed higher SOD, T-AOC, and GSH-Px, and lower MDA than group S (p < .05).
TEMPOL pretreatment attenuated postoperative cognitive impairment induced by sevoflurane anesthesia in aged rats. This may be attributed to the downregulation of NR2B-CREB-BDNF pathway, reducing the inflammatory response and oxidative stress damage in hippocampal tissue.
杂环化合物 4-羟基-(2,2,6,6-四甲基哌啶-1-基)氧自由基（TEMPOL）对缺血缺氧性脑损伤的神经功能具有保护作用。本研究探讨了 TEMPOL 预处理对老龄大鼠七氟醚麻醉后术后认知功能的影响，重点关注炎症反应和氧化应激。
60 只雄性大鼠分为正常对照组（C）、七氟醚麻醉组（S）、TEMPOL 预处理组（T）和七氟醚麻醉+TEMPOL 预处理组（ST）（每组 15 只）。T 组和 ST 组大鼠连续腹腔内注射 TEMPOL（100mg/kg）3 天，C 组和 S 组大鼠注射 0.9%生理盐水。预处理后，S 组和 ST 组大鼠接受 3%七氟醚麻醉。
S 组大鼠的游泳距离更长，逃避潜伏期更长，平台穿越频率更低，目标象限停留时间更短，与 C 组和 T 组大鼠相比。ST 组大鼠的游泳距离更短，逃避潜伏期更短，平台穿越频率更高，目标象限停留时间更长，与 S 组大鼠相比。S 组和 ST 组大鼠的白细胞介素-6、肿瘤坏死因子-α、诱导型一氧化氮合酶和 Ym1/2 信使核糖核酸表达高于 C 组和 T 组大鼠，ST 组大鼠低于 S 组大鼠（p<0.05）。S 组和 ST 组大鼠超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）和谷胱甘肽过氧化物酶（GSH-Px）水平较低，丙二醛（MDA）水平较高，与 C 组和 T 组大鼠相比（p<0.05）。ST 组 SOD、T-AOC 和 GSH-Px 水平高于 S 组，MDA 水平低于 S 组（p<0.05）。
TEMPOL 预处理可减轻老龄大鼠七氟醚麻醉后术后认知功能障碍，这可能与下调 NR2B-CREB-BDNF 通路有关，从而减轻海马组织的炎症反应和氧化应激损伤。
