Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR.
Department of Dental Surgery, University of Hong Kong-Shenzhen Hospital, Shenzhen, China.
J Endod. 2023 Dec;49(12):1634-1640. doi: 10.1016/j.joen.2023.09.011. Epub 2023 Oct 2.
This laboratory study aimed to evaluate the effect of trans-cinnamaldehyde (TC) conditioning on dentin tissue stabilization, bacterial adhesion, and stem cell toxicity.
Dentin beams (n = 204) from extracted human molars were demineralized in phosphoric acid and treated with TC (2.5, 5, and 7.5%), 50% ethanol-water mixture (vehicle control) or 2.5% glutaraldehyde (GA) (positive control) for 30 minutes. Demineralized but untreated specimens served as the negative control. After treatment, collagen crosslinking was characterized by measuring the elastic modulus (E) and hardness (n = 5). Biodegradation resistance was examined by determining the loss of dry mass (n = 8), hydroxyproline release (n = 4) and scanning electron microscopy (n = 2), after exposure to bacterial collagenase. Inhibition of bacterial adhesion was investigated by colony counting assay (n = 12) and scanning electron microscopy (n = 2). Viability of stem cells of the apical papilla on TC-conditioned dentin was determined using the Cell Counting Kit-8 assay (n = 8). Data were statistically analyzed using one-way analysis of variance (ANOVA) test followed by Dunnett's multiple comparisons at a significance level of 5%.
TC-conditioned dentin showed a concentration-dependent increase in E and hardness. The E and hardness of 5% and 7.5% TC-conditioned dentin were significantly greater than that of the negative control and vehicle control groups (P < .05). There was no significant difference in the biodegradation resistance between GA and 5% TC-conditioned dentin (P > .05). TC-conditioned dentin showed a well-preserved collagen fibril network with clear cross-banding, comparable to GA-conditioned dentin. All concentrations of TC inhibited bacterial adhesion on dentin, significantly greater than the negative control (P < .05). There was no reduction in viability of stem cells of the apical papilla viability on TC-conditioned dentin compared to the negative control (P > .05).
TC conditioning stabilized the dentin and protected it from enzymatic degradation. TC prevented bacterial adhesion on the dentin but maintained stem cell viability.
本实验室研究旨在评估肉桂醛（TC）调理对牙本质组织稳定性、细菌黏附以及干细胞毒性的影响。
从人磨牙中提取牙本质梁（n=204），用磷酸脱矿，用 TC（2.5%、5%和 7.5%）、50%乙醇-水混合物（载体对照）或 2.5%戊二醛（GA）（阳性对照）处理 30 分钟。未经处理的脱矿标本作为阴性对照。处理后，通过测量弹性模量（E）和硬度（n=5）来表征胶原交联。通过测定干重损失（n=8）、羟脯氨酸释放（n=4）和扫描电子显微镜（n=2）来检测抗生物降解能力，然后将标本暴露于细菌胶原酶中。通过菌落计数测定法（n=12）和扫描电子显微镜（n=2）研究细菌黏附的抑制作用。使用细胞计数试剂盒-8 测定法（n=8）来确定根尖乳头干细胞在 TC 调理牙本质上的活力。使用单因素方差分析（ANOVA）检验，然后在 5%的显著性水平下进行 Dunnett 多重比较，对数据进行统计学分析。
TC 调理牙本质的 E 和硬度呈浓度依赖性增加。5%和 7.5%TC 调理牙本质的 E 和硬度明显大于阴性对照组和载体对照组（P<0.05）。GA 和 5%TC 调理牙本质之间的抗生物降解能力无显著差异（P>0.05）。TC 调理牙本质显示胶原纤维原纤维网络保存良好，有清晰的横纹带，与 GA 调理牙本质相当。所有浓度的 TC 均抑制了牙本质上的细菌黏附，明显大于阴性对照组（P<0.05）。与阴性对照组相比，TC 调理牙本质上根尖乳头干细胞的活力没有降低（P>0.05）。
TC 调理稳定了牙本质并保护其免受酶降解。TC 防止了牙本质上的细菌黏附，但保持了干细胞的活力。
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