Hao Senyue, Amaral Marcello Magri, Zhou Chao
Department of Electrical & Systems Engineering, Washington University in Saint Louis, USA.
Department of Biomedical Engineering, Washington University in Saint Louis, USA.
Biomed Opt Express. 2023 Jul 6;14(8):3881-3898. doi: 10.1364/BOE.493725. eCollection 2023 Aug 1.
Motion artifacts, from such sources as heartbeats, respiration, or peristalsis, often degrade microscopic images or videos of live subjects. We have developed a method using circular optical coherence tomography (OCT) scans to track the transverse and axial motion of biological samples at speeds ranging from several micrometers per second to several centimeters per second. We achieve fast and high-precision measurements of the magnitude and direction of the sample's motion by adaptively controlling the circular scan pattern settings and applying interframe and intraframe analyses. These measurements are the basis of active motion compensation via feedback control for future microscopic and macroscopic imaging applications.
来自心跳、呼吸或蠕动等源头的运动伪影,常常会降低活体样本的微观图像或视频质量。我们开发了一种方法,利用圆形光学相干断层扫描(OCT)来追踪生物样本的横向和轴向运动,速度范围从每秒几微米到每秒几厘米。通过自适应控制圆形扫描模式设置并应用帧间和帧内分析,我们实现了对样本运动幅度和方向的快速且高精度测量。这些测量是未来微观和宏观成像应用中通过反馈控制进行主动运动补偿的基础。