Migration testing of microplastics from selected water and food containers by Raman microscopy.

The migration of microplastics (MPs) from plastic food packaging has received increasing attention. Despite numerous studies quantifying MPs released from food packaging, there is lack of systematic investigation on migration of MPs from food packages under US Food and Drug Administration (FDA)'s guidance for food contact substances. Herein, we aimed to determine the quantity and size distribution of MPs migrating from water and food plastic containers following US Food and Drug Administration (FDA)'s guidance using Raman microscopy. Six commonly used water and food containers made of polypropylene (PP), polyethylene terephthalate (PET), polystyrene (PS) were treated using distilled water and food stimulants (10% and 50% ethanol) under various conditions. A range of 23,702 to 490,330 particles per liter MPs with 77%- 92% smaller than 5 µm were detected, in which the PP food container exhibited the highest release of MPs when incubated with 50% ethanol at 130 °C for 15 min (equivalent to heating fatty food in a microwave). The temperature and food types were key attributes for elevating MP migration in general. Further comparison observed direct microwave (534,109 particles per liter) heating led to a significantly higher release of MPs compared to the FDA-suggested method (155,572 particles per liter). Part of MPs (12-63%) failed to be identified by Raman microscopy due to small particle size. Our estimation suggests that individuals might inhale up to 4511 MPs per kg per day. This research offers vital insights into MP migration from food and water containers, aiding in the development of relevant guidelines and facilitating MPs' risk assessment and management.