Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee, USA.
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
mSystems. 2023 Dec 21;8(6):e0074123. doi: 10.1128/msystems.00741-23. Epub 2023 Oct 26.
Sustainable processes for biological upcycling of plastic wastes in a circular bioeconomy are needed to promote decarbonization and reduce environmental pollution due to increased plastic consumption, incineration, and landfill storage. Strain characterization and proteomic analysis revealed the robust metabolic capabilities of to upcycle polyethylene into high-value chemicals. Significant proteome reallocation toward energy and lipid metabolisms was required for robust growth on hydrocarbons with n-hexadecane as the preferential substrate. However, an apparent over-investment in these same categories to utilize complex depolymerized plastic (DP) oil came at the expense of protein biosynthesis, limiting cell growth. Taken together, this study elucidates how activates its metabolism to utilize DP oil and establishes as a promising host for the upcycling of plastic wastes.
需要可持续的生物循环经济过程来生物回收塑料废物，以促进脱碳并减少因塑料消费、焚烧和垃圾填埋储存增加而导致的环境污染。菌株特性和蛋白质组学分析表明， 能够将聚乙烯生物回收成高价值化学品。在以正十六烷为首选底物的碳氢化合物上进行稳健生长需要对能量和脂质代谢进行显著的蛋白质组重新分配。然而，利用复杂的解聚塑料 (DP) 油时，这些相同类别明显的过度投资会牺牲蛋白质生物合成，从而限制细胞生长。总之，本研究阐明了 如何激活其代谢来利用 DP 油，并将 确立为塑料废物回收利用的有前途的宿主。
