Luo Xiao-Yu, Nie Tang-Jie, Liu Heng, Ding Xue-Fei, Huang Ying, Guo Chun-Ce, Zhang Wen-Gen
Forestry College, Jiangxi Agricultural University, Nanchang 330045, China Jiangxi Agricultural University Nanchang China.
Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization, Nanchang 330045, China Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization Nanchang China.
PhytoKeys. 2023 Oct 18;234:145-165. doi: 10.3897/phytokeys.234.108841. eCollection 2023.
Five taxa of Delphiniumsubg.Anthriscifolium have been karyologically studied through chromosome counting, chromosomal measurement, and karyotype symmetry. Each taxon that we investigated has a basic chromosome number of x = 8, D.anthriscifoliumvar.savatieri, D.anthriscifoliumvar.majus, , and were diploid with 2n = 16, while D.anthriscifoliumvar.anthriscifolium was tetraploid with 2n = 32. Monoploid chromosome sets of the investigated diploid taxa contained 1 metacentric chromosome, 3 submetacentric chromosomes, and 4 subtelocentric chromosomes. Higher interchromosomal asymmetry (CV) was present in and than in other taxa. The highest levels of intrachromosomal asymmetry (M) and heterogeneity in centromere position (CV) were found in D.anthriscifoliumvar.majus. Diploid and tetraploid genome sizes varied by 3.02-3.92 pg and 6.04-6.60 pg, respectively. Karyotype and genome size of D.anthriscifoliumvar.savatieri, D.anthriscifoliumvar.majus, , and were reported for the first time. Finally, based on cytological and morphological data, the classification of was revised.
通过染色体计数、染色体测量和核型对称性分析，对翠雀属髯毛翠雀亚组的五个分类群进行了核型研究。我们所研究的每个分类群的基本染色体数均为x = 8，髯毛翠雀萨瓦蒂变种、髯毛翠雀大花变种、髯毛翠雀髯毛变种和髯毛翠雀长梗变种为二倍体，2n = 16，而髯毛翠雀原变种为四倍体，2n = 32。所研究的二倍体分类群的单倍体染色体组包含1条中着丝粒染色体、3条近中着丝粒染色体和4条近端着丝粒染色体。髯毛翠雀长梗变种和髯毛翠雀髯毛变种的染色体间不对称性（CV）高于其他分类群。在髯毛翠雀大花变种中发现了最高水平的染色体内不对称性（M）和着丝粒位置的异质性（CV）。二倍体和四倍体的基因组大小分别在3.02 - 3.92 pg和6.04 - 6.60 pg之间变化。首次报道了髯毛翠雀萨瓦蒂变种、髯毛翠雀大花变种、髯毛翠雀髯毛变种和髯毛翠雀长梗变种的核型和基因组大小。最后，基于细胞学和形态学数据，对髯毛翠雀长梗变种的分类进行了修订。
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