对福氏（紫金牛科）叶80%甲醇提取物及溶剂萃取物在小鼠体内抗腹泻活性的评估
Evaluation of Anti-Diarrheal Activities of the 80% Methanol Extract and Solvent Fractions of Forssk (Myrsinaceae) Leaves in Mice.
作者信息
Megersa Alemayehu, Dereje Beyene, Adugna Meaza, Ayalew Getahun Kefyalew, Birru Eshetie Melese
机构信息
Department of Pharmacology, School of Medicine, College of Medicine and Health Science, Dire Dawa University, Dire Dawa, Ethiopia.
Department of Pharmacology, School of Pharmacy, College of Medicine and Health Science, University of Gondar, Gondar, Ethiopia.
出版信息
J Exp Pharmacol. 2023 Oct 25;15:391-405. doi: 10.2147/JEP.S429403. eCollection 2023.
BACKGROUND
Due to the limits of present antidiarrheal medications, it is critical to seek novel, safe, and inexpensive antidiarrheal agents. Thus, the goal of this study was to assess the antidiarrheal activity of 80% methanol crude extract and solvent fractions of leaves in mice.
METHODS
Leaf powder was extracted by 80% methanol and then fractionated with n-hexane, ethyl acetate, and distilled water. At 100, 200, and 400 mg/kg, the effects of the crude extract on castor oil-induced diarrhea, enteropooling, and gastrointestinal motility tests were investigated. Tween 2% and atropine used as negative and positive controls, respectively. A gastrointestinal motility test was used to explore the anti-motility effects. Data were analyzed with SPSS V. 26, and the significance was established with a one-way ANOVA followed by a post hoc Tukey's test.
RESULTS
The crude extract delayed the onset of diarrhea and significantly reduced the number of fecal drops at 100 (p<0.05), 200 and 400 mg/kg (p<0.001). Similarly, the number and weight of wet feces, as well as total fresh feces, were reduced at 200 (p<0.05) and 400 mg/kg (p<0.001) compared to Tween 2%. The enteropooling test demonstrated that the extracts significantly reduced the volume and weight of intestine content at 200 (p<0.05) and 400 mg/kg (p<0.001). The anti-motility activity test revealed that the all extracts decreased gastrointestinal motility significantly (p<0.001). The ethyl acetate fraction significantly reduced gastrointestinal transit time at all doses (p<0.001). At 400 mg/kg, the activities of the n-hexane fraction were significant (p<0.01). The efficacy of the residual aqueous fraction on gastrointestinal motility was significant at 200 (p<0.05) and 400 mg/kg (p<0.001).
CONCLUSION
The 80% methanol extract of Forssk leaf and solvent fractions were shown to exhibit potent antidiarrheal activity in the current study.
背景
由于目前抗腹泻药物存在局限性，寻找新型、安全且廉价的抗腹泻药物至关重要。因此，本研究的目的是评估 Forssk 叶的 80%甲醇粗提物及其溶剂萃取物在小鼠中的抗腹泻活性。
方法
叶粉用 80%甲醇提取，然后依次用正己烷、乙酸乙酯和蒸馏水进行萃取。在 100、200 和 400 mg/kg 剂量下，研究粗提物对蓖麻油诱导的腹泻、肠积液和胃肠动力试验的影响。分别以 2%吐温溶液和阿托品作为阴性和阳性对照。采用胃肠动力试验来探究抗动力作用。数据用 SPSS V. 26 进行分析，显著性通过单因素方差分析和事后 Tukey 检验确定。
结果
粗提物延迟了腹泻发作，在 100 mg/kg（p<0.05）、200 和 400 mg/kg（p<0.001）时显著减少了粪便滴数。同样，与 2%吐温溶液相比，在 200 mg/kg（p<0.05）和 400 mg/kg（p<0.001）时，湿粪便的数量和重量以及总新鲜粪便量均有所减少。肠积液试验表明，提取物在 200 mg/kg（p<0.05）和 400 mg/kg（p<0.001）时显著降低了肠内容物的体积和重量。抗动力活性试验显示，所有提取物均显著降低了胃肠动力（p<0.001）。乙酸乙酯萃取物在所有剂量下均显著缩短了胃肠转运时间（p<0.001）。在 400 mg/kg 时，正己烷萃取物的活性显著（p<0.01）。残留水相萃取物在 200 mg/kg（p<0.05）和 400 mg/kg（p<0.001）时对胃肠动力的作用显著。
结论
本研究表明，Forssk 叶的 80%甲醇提取物及其溶剂萃取物具有显著的抗腹泻活性。
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