Wang Xiaoyan, Qiao Yinuo, Zhang Jinyang, Song Yuzhu, Han Qinqin
Engineering Research Center for Molecular Diagnosis, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.
Anal Bioanal Chem. 2024 Jan;416(1):299-311. doi: 10.1007/s00216-023-05018-6. Epub 2023 Nov 7.
Methylmercury (MeHg) is a common form of organic mercury that is substantially more toxic than inorganic mercury and is more likely to accumulate in organisms through biological enrichment. Therefore, developing a method to enable the specific and rapid detection of MeHg in seafood is important and remains challenging to accomplish. Herein, a rapid, label-free fluorescence detection method for MeHg determination was developed based on SYBR Green I. The detection system implemented "add and measure" detection mode can be completed in 10 min. Under optimal assay conditions, the detection platform showed a linear relationship with the concentration of MeHg within 1-50 nM (Y = 8.573x + 42.89, R = 0.9928), with a detection limit of 0.3218 nM. The results obtained for competitive substances, such as inorganic mercury ions and anions, show a high specificity of the method. In addition, this method successfully detected MeHg in seawater and marine products, with an accompanying spike recovery rate of 96.45-105.1%.
甲基汞（MeHg）是有机汞的一种常见形式，其毒性远高于无机汞，并且更有可能通过生物富集在生物体中积累。因此，开发一种能够特异性快速检测海鲜中甲基汞的方法非常重要，且仍具有挑战性。在此，基于SYBR Green I开发了一种用于测定甲基汞的快速、无标记荧光检测方法。实施“添加并测量”检测模式的检测系统可在10分钟内完成。在最佳检测条件下，检测平台在1-50 nM范围内与甲基汞浓度呈线性关系（Y = 8.573x + 42.89，R = 0.9928），检测限为0.3218 nM。无机汞离子和阴离子等竞争物质的检测结果表明该方法具有高特异性。此外，该方法成功检测了海水中和海产品中的甲基汞，加标回收率为96.45-105.1%。
Zotero 插件