Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, Maine, USA.
School of Food and Agriculture, University of Maine, Orono, Maine, USA.
mSystems. 2023 Dec 21;8(6):e0068823. doi: 10.1128/msystems.00688-23. Epub 2023 Nov 9.
To our knowledge, IL-10-KO mice have not previously been used to investigate the interactions of host, microbiota, and broccoli, broccoli sprouts, or broccoli bioactives in resolving symptoms of CD. We showed that a diet containing 10% raw broccoli sprouts increased the plasma concentration of the anti-inflammatory compound sulforaphane and protected mice to varying degrees against disease symptoms, including weight loss or stagnation, fecal blood, and diarrhea. Younger mice responded more strongly to the diet, further reducing symptoms, as well as increased gut bacterial richness, increased bacterial community similarity to each other, and more location-specific communities than older mice on the diet intervention. Crohn's disease disrupts the lives of patients and requires people to alter dietary and lifestyle habits to manage symptoms. The current medical treatment is expensive with significant side effects, and a dietary intervention represents an affordable, accessible, and simple strategy to reduce the burden of symptoms.
据我们所知，此前尚未有研究使用 IL-10-KO 小鼠来研究宿主、微生物组与西兰花、西兰花芽或西兰花生物活性物质之间的相互作用，以缓解 CD 症状。我们发现，含有 10%生西兰花芽的饮食可增加抗炎化合物萝卜硫素的血浆浓度，并在不同程度上保护小鼠免受疾病症状的影响，包括体重减轻或停滞、粪便带血和腹泻。年轻小鼠对饮食的反应更强烈，进一步减轻了症状，同时增加了肠道细菌丰富度、增加了细菌群落之间的相似性，以及比年长小鼠更多的特定于位置的群落。克罗恩病会扰乱患者的生活，需要人们改变饮食和生活习惯来控制症状。目前的医疗费用昂贵，且副作用明显，而饮食干预是一种负担得起、可及且简单的策略，可以减轻症状的负担。
